Name: Anti-VLDLR Antibody (CAB7345)
Brand: Metabolism Antibodies 2
SKU: CAB7345
Availability: OutOfStock

Antibody Name:	Anti-VLDLR Antibody
Antibody SKU:	CAB7345
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-210 of human VLDLR (NP_001018066.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	THP-1, Mouse lung, Mouse ovary

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-210 of human VLDLR (NP_001018066.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	RESG ATGT GRKA KCEP SQFQ CTNG RCIT LLWK CDGD EDCV DGSD EKNC VKKT CAES DFVC NNGQ CVPS RWKC DGDP DCED GSDE SPEQ CHMR TCRI HEIS CGAH STQC IPVS WRCD GEND CDSG EDEE NCGN ITCS PDEF TCSS GRCI SRNF VCNG QDDC SDGS DELD CAPP TCGA HEFQ CSTS SCIP ISW
Gene ID:	7436
Uniprot:	P98155
Cellular Location:	Membrane, Single-pass type I membrane protein, clathrin-coated pit
Calculated MW:	93kDa/96kDa
Observed MW:	110kDa

Synonyms:

VLDLR, CAMRQ1, CARMQ1, CHRMQ1, VLDL-R, VLDLRCH

Background:

The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. This gene encodes a lipoprotein receptor that is a member of the LDLR family and plays important roles in VLDL-triglyceride metabolism and the reelin signaling pathway. Mutations in this gene cause VLDLR-associated cerebellar hypoplasia. Alternative splicing generates multiple transcript variants encoding distinct isoforms for this gene.

UniProt Protein Function:	VLDLR: Binds VLDL and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. Binding to Reelin induces tyrosine phosphorylation of Dab1 and modulation of Tau phosphorylation. Defects in VLDLR are the cause of cerebellar ataxia mental retardation and dysequilibrium syndrome type 1 (CMARQ1); also known as dysequilibrium syndrome (DES) or non- progressive cerebellar disorder with mental retardation. CMARQ1 is a congenital, non-progressive cerebellar ataxia associated with disturbed equilibrium, delayed ambulation, mental retardation and cerebellar hypoplasia. Additional features include short stature, strabismus, pes planus and, rarely, seizures. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, integral; Receptor, misc. Chromosomal Location of Human Ortholog: 9p24 Cellular Component: membrane; plasma membrane; integral to membrane; coated pit; receptor complex Molecular Function:very-low-density lipoprotein receptor activity; low-density lipoprotein receptor activity; very-low-density lipoprotein binding; protein binding; apolipoprotein binding; calcium ion binding; glycoprotein binding; calcium-dependent protein binding Biological Process: nervous system development; receptor-mediated endocytosis; cholesterol metabolic process; positive regulation of protein kinase activity; ventral spinal cord development; negative regulation of transcription from RNA polymerase II promoter; signal transduction; lipid transport; memory Disease: Cerebellar Ataxia, Mental Retardation, And Dysequilibrium Syndrome 1
NCBI Summary:	The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. This gene encodes a lipoprotein receptor that is a member of the LDLR family and plays important roles in VLDL-triglyceride metabolism and the reelin signaling pathway. Mutations in this gene cause VLDLR-associated cerebellar hypoplasia. Alternative splicing generates multiple transcript variants encoding distinct isoforms for this gene. [provided by RefSeq, Aug 2009]
UniProt Code:	P98155
NCBI GenInfo Identifier:	65301167
NCBI Gene ID:	7436
NCBI Accession:	NP_003374.3
UniProt Secondary Accession:	P98155,Q5VVF6, B2RMZ7, D3DRH6,
UniProt Related Accession:	P98155
Molecular Weight:	96,098 Da
NCBI Full Name:	very low-density lipoprotein receptor isoform a
NCBI Synonym Full Names:	very low density lipoprotein receptor
NCBI Official Symbol:	VLDLR
NCBI Official Synonym Symbols:	CARMQ1; CHRMQ1; VLDLRCH
NCBI Protein Information:	very low-density lipoprotein receptor; VLDL-R; VLDL receptor
UniProt Protein Name:	Very low-density lipoprotein receptor
Protein Family:	Very low-density lipoprotein receptor
UniProt Gene Name:	VLDLR
UniProt Entry Name:	VLDLR_HUMAN

Western blot analysis of extracts of various cell lines, using VLDLR antibody (CAB7345) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.

ELISA Kits

Human VLDL R / Very low-density lipoprotein receptor ELISA Kit

Human VLDL / Very low-density lipoprotein ELISA Kit

Human Very low-density lipoprotein receptor (VLDLR) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)