Antibody Name:	Anti-VDAC3 Antibody
Antibody SKU:	CAB10544
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-283 of human VDAC3 (NP_005653.3).

Application:	WB IF
Recommended Dilution:	WB 1:1000 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human
Positive Samples:	A-431

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-283 of human VDAC3 (NP_005653.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MCNT PTYC DLGK AAKD VFNK GYGF GMVK IDLK TKSC SGVE FSTS GHAY TDTG KASG NLET KYKV CNYG LTFT QKWN TDNT LGTE ISWE NKLA EGLK LTLD TIFV PNTG KKSG KLKA SYKR DCFS VGSN VDID FSGP TIYG WAVL AFEG WLAG YQMS FDTA KSKL SQNN FALG YKAA DFQL HTHV NDGT EFGG SIYQ KVNE KIET SINL AWTA GSNN TRFG IAAK YMLD CRTS LSAK VNNA SLIG LGYT QTLR PGVK LTLS ALID GKNF SAGG HKVG LGFE LEA
Gene ID:	7419
Uniprot:	Q9Y277
Cellular Location:	Mitochondrion outer membrane
Calculated MW:	30kDa
Observed MW:	31kDa

Synonyms:

VDAC3, HD-VDAC3, VDAC-3

Background:

This gene encodes a voltage-dependent anion channel (VDAC), and belongs to the mitochondrial porin family. VDACs are small, integral membrane proteins that traverse the outer mitochondrial membrane and conduct ATP and other small metabolites. They are known to bind several kinases of intermediary metabolism, thought to be involved in translocation of adenine nucleotides, and are hypothesized to form part of the mitochondrial permeability transition pore, which results in the release of cytochrome c at the onset of apoptotic cell death. Alternatively transcript variants encoding different isoforms have been described for this gene.

UniProt Protein Function:	VDAC3: a protein of the eukaryotic mitochondrial porin family. Forms a voltage-dependent anion channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. Two splice-variant isoforms have been described.
UniProt Protein Details:	Protein type:Channel, misc.; Membrane protein, integral; Mitochondrial Chromosomal Location of Human Ortholog: 8p11.21 Cellular Component: mitochondrion; nucleus Molecular Function:voltage-gated anion channel activity Biological Process: adenine transport
NCBI Summary:	This gene encodes a voltage-dependent anion channel (VDAC), and belongs to the mitochondrial porin family. VDACs are small, integral membrane proteins that traverse the outer mitochondrial membrane and conduct ATP and other small metabolites. They are known to bind several kinases of intermediary metabolism, thought to be involved in translocation of adenine nucleotides, and are hypothesized to form part of the mitochondrial permeability transition pore, which results in the release of cytochrome c at the onset of apoptotic cell death. Alternatively transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011]
UniProt Code:	Q9Y277
NCBI GenInfo Identifier:	12643945
NCBI Gene ID:	7419
NCBI Accession:	Q9Y277.1
UniProt Secondary Accession:	Q9Y277,Q9UIS0,
UniProt Related Accession:	Q9Y277
Molecular Weight:	31kDa
NCBI Full Name:	Voltage-dependent anion-selective channel protein 3
NCBI Synonym Full Names:	voltage dependent anion channel 3
NCBI Official Symbol:	VDAC3
NCBI Official Synonym Symbols:	VDAC-3; HD-VDAC3
NCBI Protein Information:	voltage-dependent anion-selective channel protein 3
UniProt Protein Name:	Voltage-dependent anion-selective channel protein 3
UniProt Synonym Protein Names:	Outer mitochondrial membrane protein porin 3
Protein Family:	Voltage-dependent anion-selective channel protein
UniProt Gene Name:	VDAC3

	Western blot analysis of extracts of A-431 cells, using VDAC3 antibody (CAB10544) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunofluorescence analysis of HeLa cells using VDAC3 antibody (CAB10544) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.