Cell Biology Antibodies 5

Anti-VAV1 Antibody (CAB15108)

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SKU:
CAB15108
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-VAV1 Antibody
Antibody SKU:CAB15108
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 100-200 of human VAV1 (NP_005419.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:Jurkat
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 100-200 of human VAV1 (NP_005419.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:FDVQ DFGK VIYT LSAL SWTP IAQN RGIM PFPT EEES VGDE DIYS GLSD QIDD TVEE DEDL YDCV ENEE AEGD EIYE DLMR SEPV SMPP KMTE YDKR CCCL R
Gene ID:7409
Uniprot:P15498
Cellular Location:
Calculated MW:94kDa/98kDa
Observed MW:110kDa
Synonyms:VAV1, VAV
Background:This gene is a member of the VAV gene family. The VAV proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases that activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. The encoded protein is important in hematopoiesis, playing a role in T-cell and B-cell development and activation. The encoded protein has been identified as the specific binding partner of Nef proteins from HIV-1. Coexpression and binding of these partners initiates profound morphological changes, cytoskeletal rearrangements and the JNK/SAPK signaling cascade, leading to increased levels of viral transcription and replication. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
UniProt Protein Function:VAV1: a proto-oncogenic guanine nucleotide exchange factors (GEF) of the Dbl family. A GEF for the Rho family of GTP binding proteins. Expressed exclusively in hematopoietic cells. Important in hematopoiesis, playing a role in T-cell and B-cell development and activation.
UniProt Protein Details:

Protein type:GEFs, Rac/Rho; GEFs; Oncoprotein; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: plasma membrane; intercellular junction; cytosol

Molecular Function:protein binding; metal ion binding; Rac guanyl-nucleotide exchange factor activity; guanyl-nucleotide exchange factor activity; transcription factor activity

Biological Process: epidermal growth factor receptor signaling pathway; integrin-mediated signaling pathway; neutrophil chemotaxis; platelet activation; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; T cell activation; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; G-protein coupled receptor protein signaling pathway; regulation of small GTPase mediated signal transduction; regulation of transcription, DNA-dependent; small GTPase mediated signal transduction; T cell costimulation; innate immune response; blood coagulation; vascular endothelial growth factor receptor signaling pathway

NCBI Summary:This gene is a member of the VAV gene family. The VAV proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases that activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. The encoded protein is important in hematopoiesis, playing a role in T-cell and B-cell development and activation. The encoded protein has been identified as the specific binding partner of Nef proteins from HIV-1. Coexpression and binding of these partners initiates profound morphological changes, cytoskeletal rearrangements and the JNK/SAPK signaling cascade, leading to increased levels of viral transcription and replication. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Apr 2012]
UniProt Code:P15498
NCBI GenInfo Identifier:13124807
NCBI Gene ID:7409
NCBI Accession:P15498.4
UniProt Secondary Accession:P15498,Q15860, B4DVK9, M0QXX6,
UniProt Related Accession:P15498
Molecular Weight:845
NCBI Full Name:Proto-oncogene vav
NCBI Synonym Full Names:vav 1 guanine nucleotide exchange factor
NCBI Official Symbol:VAV1  
NCBI Official Synonym Symbols:VAV  
NCBI Protein Information:proto-oncogene vav; vav 1 oncogene
UniProt Protein Name:Proto-oncogene vav
Protein Family:Proto-oncogene
UniProt Gene Name:VAV1  
UniProt Entry Name:VAV_HUMAN
Anti-VAV1 Antibody (CAB15108)Western blot analysis of extracts of Jurkat cells, using VAV1 antibody (CAB15108) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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