Metabolism Antibodies 1

Anti-VARS2 Antibody (CAB15484)

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SKU:
CAB15484
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-VARS2 Antibody
Antibody SKU:CAB15484
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 864-1063 of human VARS2 (NP_065175.4).
Application:WB
Recommended Dilution:WB 1:200 - 1:2000
Reactivity:Human
Positive Samples:U-251MG
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 864-1063 of human VARS2 (NP_065175.4).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LWQR LPPR PGCP PAPS ISVA PYPS ACSL EHWR QPEL ERRF SRVQ EVVQ VLRA LRAT YQLT KARP RVLL QSSE PGDQ GLFE AFLE PLGT LGYC GAVG LLPP GAAA PSGW AQAP LSDT AQVY MELQ GLVD PQIQ LPLL AARR YKLQ KQLD SLTA RTPS EGEA GTQR QQKL SSLQ LELS KLDK AASH LRQL MDEP PAPG SPEL
Gene ID:57176
Uniprot:Q5ST30
Cellular Location:Mitochondrion
Calculated MW:55kDa/102kDa/118kDa/121kDa
Observed MW:120kDa
Synonyms:VARS2, COXPD20, VALRS, VARS2L, VARSL
Background:This gene encodes a mitochondrial aminoacyl-tRNA synthetase, which catalyzes the attachment of valine to tRNA(Val) for mitochondrial translation. Mutations in this gene cause combined oxidative phosphorylation deficiency-20, and are also associated with early-onset mitochondrial encephalopathies. Alternative splicing of this gene results in multiple transcript variants.
UniProt Protein Function:VARS2: Belongs to the class-I aminoacyl-tRNA synthetase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Translation; EC 6.1.1.9; Amino Acid Metabolism - valine, leucine and isoleucine biosynthesis; Ligase

Chromosomal Location of Human Ortholog: 6p21.33

Cellular Component: cytosol

Molecular Function:valine-tRNA ligase activity

Biological Process: valyl-tRNA aminoacylation

Disease: Combined Oxidative Phosphorylation Deficiency 20

NCBI Summary:This gene encodes a mitochondrial aminoacyl-tRNA synthetase, which catalyzes the attachment of valine to tRNA(Val) for mitochondrial translation. Mutations in this gene cause combined oxidative phosphorylation deficiency-20, and are also associated with early-onset mitochondrial encephalopathies. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Aug 2014]
UniProt Code:Q5ST30
NCBI GenInfo Identifier:109731090
NCBI Gene ID:57176
NCBI Accession:BC113605
UniProt Secondary Accession:Q5ST30,Q2M2A0, Q59FI1, Q5SQ96, Q5SS98, Q6DKJ5, Q6ZV24 A2ABL7, B4DET4, B4E3P5, F5GXJ0, F5H323,
UniProt Related Accession:Q5ST30
Molecular Weight:121,543 Da
NCBI Full Name:Homo sapiens valyl-tRNA synthetase 2, mitochondrial (putative), mRNA
NCBI Synonym Full Names:valyl-tRNA synthetase 2, mitochondrial
NCBI Official Symbol:VARS2  
NCBI Official Synonym Symbols:VALRS; VARSL; VARS2L; COXPD20  
NCBI Protein Information:valine--tRNA ligase, mitochondrial
UniProt Protein Name:Valine--tRNA ligase, mitochondrial
UniProt Synonym Protein Names:Valyl-tRNA synthetase; ValRS; Valyl-tRNA synthetase-like
UniProt Gene Name:VARS2  
UniProt Entry Name:SYVM_HUMAN
Anti-VARS2 Antibody (CAB15484)Western blot analysis of extracts of U-251MG cells, using VARS2 antibody (CAB15484) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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