Immunology Antibodies 2
Anti-VAPB Antibody (CAB5363)
- SKU:
- CAB5363
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-VAPB Antibody |
| Antibody SKU: | CAB5363 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 10-222 of human VAPB (NP_004729.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:20 - 1:50 |
| Reactivity: | Human, Mouse |
| Positive Samples: | BT-474, SW480, HepG2, NCI-H460, Raji, Mouse brain, Mouse intestine |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 10-222 of human VAPB (NP_004729.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LEPQ HELK FRGP FTDV VTTN LKLG NPTD RNVC FKVK TTAP RRYC VRPN SGII DAGA SINV SVML QPFD YDPN EKSK HKFM VQSM FAPT DTSD MEAV WKEA KPED LMDS KLRC VFEL PAEN DKPH DVEI NKII STTA SKTE TPIV SKSL SSSL DDTE VKKV MEEC KRLQ GEVQ RLRE ENKQ FKEE DGLR MRKT VQSN SPIS ALAP TGKE EGLS T |
| Gene ID: | 9217 |
| Uniprot: | O95292 |
| Cellular Location: | Endoplasmic reticulum membrane, Single-pass type IV membrane protein |
| Calculated MW: | 11kDa/27kDa |
| Observed MW: | 35kDa/47kDa |
| Synonyms: | VAPB, ALS8, VAMP-B, VAP-B |
| Background: | The protein encoded by this gene is a type IV membrane protein found in plasma and intracellular vesicle membranes. The encoded protein is found as a homodimer and as a heterodimer with VAPA. This protein also can interact with VAMP1 and VAMP2 and may be involved in vesicle trafficking. |
| UniProt Protein Function: | VAPB: Participates in the endoplasmic reticulum unfolded protein response (UPR) by inducing ERN1/IRE1 activity. Involved in cellular calcium homeostasis regulation. Defects in VAPB are the cause of amyotrophic lateral sclerosis type 8 (ALS8). ALS8 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms. Defects in VAPB are a cause of spinal muscular atrophy proximal adult autosomal dominant (SMAPAD); also called late-onset spinal muscular atrophy Finkel type. A form of spinal muscular atrophy, a neuromuscular disorder characterized by degeneration of the anterior horn cells of the spinal cord, leading to symmetrical muscle weakness and atrophy. SMAPAD is characterized by proximal muscle weakness that begins in the lower limbs and then progresses to upper limbs, onset in late adulthood (after third decade) and a benign course. Most of the patients remain ambulatory 10 to 40 years after clinical onset. Belongs to the VAMP-associated protein (VAP) (TC 9.B.17) family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 20q13.33 Cellular Component: Golgi membrane; Golgi apparatus; endoplasmic reticulum membrane; endoplasmic reticulum; integral to membrane Molecular Function:protein binding; enzyme binding; protein homodimerization activity; FFAT motif binding; protein heterodimerization activity; microtubule binding; beta-tubulin binding Biological Process: endoplasmic reticulum organization and biogenesis; cellular calcium ion homeostasis; ER to Golgi vesicle-mediated transport; sphingolipid metabolic process; positive regulation of viral genome replication; unfolded protein response, activation of signaling protein activity; sphingolipid biosynthetic process; unfolded protein response; virus-host interaction; negative regulation of viral protein levels in host cell Disease: Spinal Muscular Atrophy, Late-onset, Finkel Type; Amyotrophic Lateral Sclerosis 8 |
| NCBI Summary: | The protein encoded by this gene is a type IV membrane protein found in plasma and intracellular vesicle membranes. The encoded protein is found as a homodimer and as a heterodimer with VAPA. This protein also can interact with VAMP1 and VAMP2 and may be involved in vesicle trafficking. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O95292 |
| NCBI GenInfo Identifier: | 24638339 |
| NCBI Gene ID: | 9217 |
| NCBI Accession: | O95292.3 |
| UniProt Secondary Accession: | O95292,O95293, Q9P0H0, A2A2F2, |
| UniProt Related Accession: | O95292 |
| Molecular Weight: | 243 |
| NCBI Full Name: | Vesicle-associated membrane protein-associated protein B/C |
| NCBI Synonym Full Names: | VAMP (vesicle-associated membrane protein)-associated protein B and C |
| NCBI Official Symbol: | VAPB |
| NCBI Official Synonym Symbols: | ALS8; VAP-B; VAMP-B |
| NCBI Protein Information: | vesicle-associated membrane protein-associated protein B/C; VAMP-associated 33 kDa protein |
| UniProt Protein Name: | Vesicle-associated membrane protein-associated protein B/C |
| Protein Family: | Virulence-associated protein |
| UniProt Gene Name: | VAPB |
| UniProt Entry Name: | VAPB_HUMAN |
 | Western blot analysis of extracts of various cell lines, using VAPB antibody (CAB5363) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunohistochemistry of paraffin-embedded human liver cancer using VAPB Antibody (CAB5363) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human kidney cancer using VAPB Antibody (CAB5363) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse brain using VAPB Antibody (CAB5363) at dilution of 1:200 (40x lens). |
 | Immunofluorescence analysis of HeLa cells using VAPB antibody (CAB5363). Blue: DAPI for nuclear staining. |
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