Antibody Name:	Anti-VAPA Antibody
Antibody SKU:	CAB12939
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 137-227 of human VAPA (NP_919415.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	B-cell, Mouse heart, Mouse pancreas

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 137-227 of human VAPA (NP_919415.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	DKLN DMEP SKAV PLNA SKQD GPMP KPHS VSLN DTET RKLM EECK RLQG EMMK LSEE NRHL RDEG LRLR KVAH SDKP GSTS TASF RDNV TSP
Gene ID:	9218
Uniprot:	Q9P0L0
Cellular Location:	Endoplasmic reticulum membrane, Single-pass type IV membrane protein
Calculated MW:	27kDa/32kDa
Observed MW:	28kDa

Synonyms:

VAPA, VAP-33, VAP-A, VAP33, hVAP-33

Background:

The protein encoded by this gene is a type IV membrane protein. It is present in the plasma membrane and intracellular vesicles. It may also be associated with the cytoskeleton. This protein may function in vesicle trafficking, membrane fusion, protein complex assembly and cell motility. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified.

UniProt Protein Function:	VAPA: May play a role in vesicle trafficking. Belongs to the VAMP-associated protein (VAP) (TC 9.B.17) family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Endoplasmic reticulum; Membrane protein, integral Chromosomal Location of Human Ortholog: 18p11.22 Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; perinuclear region of cytoplasm; plasma membrane; tight junction; vesicle Molecular Function:FFAT motif binding; microtubule binding; protein binding; protein domain specific binding; protein heterodimerization activity; signal transducer activity Biological Process: cell death; endoplasmic reticulum organization and biogenesis; ER to Golgi vesicle-mediated transport; neurite development; positive regulation of I-kappaB kinase/NF-kappaB cascade; sphingolipid biosynthetic process
NCBI Summary:	The protein encoded by this gene is a type IV membrane protein. It is present in the plasma membrane and intracellular vesicles. It may also be associated with the cytoskeleton. This protein may function in vesicle trafficking, membrane fusion, protein complex assembly and cell motility. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9P0L0
NCBI GenInfo Identifier:	122066680
NCBI Gene ID:	9218
NCBI Accession:	Q9P0L0.3
UniProt Secondary Accession:	Q9P0L0,O75453, Q5U0E7, Q9UBZ2, A6NDZ0, D3DUI3,
UniProt Related Accession:	Q9P0L0
Molecular Weight:	32,614 Da
NCBI Full Name:	Vesicle-associated membrane protein-associated protein A
NCBI Synonym Full Names:	VAMP associated protein A
NCBI Official Symbol:	VAPA
NCBI Official Synonym Symbols:	VAP-A; VAP33; VAP-33; hVAP-33
NCBI Protein Information:	vesicle-associated membrane protein-associated protein A
UniProt Protein Name:	Vesicle-associated membrane protein-associated protein A
UniProt Synonym Protein Names:	33 kDa VAMP-associated protein; VAP-33
Protein Family:	Virulence-associated protein
UniProt Gene Name:	VAPA
UniProt Entry Name:	VAPA_HUMAN

	Western blot analysis of extracts of various cell lines, using VAPA Antibody (CAB12939) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human stomach using VAPA antibody (CAB12939) at dilution of 1:100 (40x lens).