Antibody Name:	Anti-VAMP1 Antibody
Antibody SKU:	CAB8877
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-96 of human VAMP1 (NP_954740.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse brain, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-96 of human VAMP1 (NP_954740.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSAP AQPP AEGT EGTA PGGG PPGP PPNM TSNR RLQQ TQAQ VEEV VDII RVNV DKVL ERDQ KLSE LDDR ADAL QAGA SQFE SSAA KLKR KYWW KNCK
Gene ID:	6843
Uniprot:	P23763
Cellular Location:	Cell junction, Cytoplasmic vesicle, Cytoplasmic vesicle membrane, Mitochondrion outer membrane, Single-pass type IV membrane protein, secretory vesicle, synapse, synaptic vesicle membrane, synaptosome, synaptosome
Calculated MW:	12kDa
Observed MW:	17kDa

Synonyms:

VAMP1, SPAX1, SYB1, VAMP-1

Background:

Synapotobrevins, syntaxins, and the synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Mutations in this gene are associated with autosomal dominant spastic ataxia 1. Multiple alternative splice variants have been described, but the full-length nature of some variants has not been defined.

UniProt Protein Function:	VAMP1: Involved in the targeting and/or fusion of transport vesicles to their target membrane. Belongs to the synaptobrevin family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 12p Cellular Component: cell junction; cell surface; cytosol; integral to plasma membrane; mitochondrial outer membrane; synaptic vesicle membrane; terminal button Molecular Function:protein binding; SNAP receptor activity; SNARE binding Biological Process: vesicle fusion Disease: Spastic Ataxia 1, Autosomal Dominant
NCBI Summary:	Synapotobrevins, syntaxins, and the synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Mutations in this gene are associated with autosomal dominant spastic ataxia 1. Multiple alternative splice variants have been described, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2014]
UniProt Code:	P23763
NCBI GenInfo Identifier:	135093
NCBI Gene ID:	6843
NCBI Accession:	P23763.1
UniProt Secondary Accession:	P23763,O75468, Q15857, Q6FG94, Q8IVC9, A8MVP3, D3DUR3
UniProt Related Accession:	P23763
Molecular Weight:	12,658 Da
NCBI Full Name:	Vesicle-associated membrane protein 1
NCBI Synonym Full Names:	vesicle associated membrane protein 1
NCBI Official Symbol:	VAMP1
NCBI Official Synonym Symbols:	SYB1; SPAX1; VAMP-1
NCBI Protein Information:	vesicle-associated membrane protein 1
UniProt Protein Name:	Vesicle-associated membrane protein 1
UniProt Synonym Protein Names:	Synaptobrevin-1
Protein Family:	Vesicle-associated membrane protein
UniProt Gene Name:	VAMP1
UniProt Entry Name:	VAMP1_HUMAN

	Western blot analysis of extracts of various cell lines, using VAMP1 antibody (CAB8877) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded rat ovary using VAMP1 antibody (CAB8877) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat spleen using VAMP1 antibody (CAB8877) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human gastric cancer using VAMP1 antibody (CAB8877) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse lung using VAMP1 antibody (CAB8877) at dilution of 1:100 (40x lens).