Cell Biology Antibodies 4
Anti-USP53 Antibody (CAB14353)
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- SKU:
- CAB14353
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-USP53 Antibody |
| Antibody SKU: | CAB14353 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 886-1073 of human USP53 (NP_061923.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | U-87MG, 293T, A-431 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 886-1073 of human USP53 (NP_061923.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MDQC YFEN SLST ECII RSAS RSDG CQMP KLFC QNLP PPLP PKKY AITS VPQS EKSE STPD VKLT EVFK ATSH LPKH SLST ASEP SLEV STHM NDER HKET FQVR ECFG NTPN CPSS SSTN DFQA NSGA IDAF CQPE LDSI STCP NETV SLTT YFSV DSCM TDTY RLKY HQRP KLSF PESS GFCN NSLS |
| Gene ID: | 54532 |
| Uniprot: | Q70EK8 |
| Cellular Location: | |
| Calculated MW: | 120kDa |
| Observed MW: | 120kDa |
| Synonyms: | USP53 |
| Background: |
| UniProt Protein Function: | USP53: Has no peptidase activity. Belongs to the peptidase C19 family. |
| UniProt Protein Details: | Protein type:Ubiquitin-specific protease Chromosomal Location of Human Ortholog: 4q26 Biological Process: ubiquitin-dependent protein catabolic process |
| UniProt Code: | Q70EK8 |
| NCBI GenInfo Identifier: | 16924225 |
| NCBI Gene ID: | 54532 |
| NCBI Accession: | AAH17382.1 |
| UniProt Secondary Accession: | Q70EK8,Q68DA5, Q8WVQ5, Q9P2J7, |
| UniProt Related Accession: | Q70EK8 |
| Molecular Weight: | 120,806 Da |
| NCBI Full Name: | USP53 protein |
| NCBI Synonym Full Names: | ubiquitin specific peptidase 53 |
| NCBI Official Symbol: | USP53 |
| NCBI Protein Information: | inactive ubiquitin carboxyl-terminal hydrolase 53; USP53; inactive ubiquitin-specific peptidase 53; ubiquitin specific protease 53; ubiquitin specific proteinase 53 |
| UniProt Protein Name: | Inactive ubiquitin carboxyl-terminal hydrolase 53 |
| UniProt Synonym Protein Names: | Inactive ubiquitin-specific peptidase 53 |
| Protein Family: | Inactive ubiquitin carboxyl-terminal hydrolase |
| UniProt Gene Name: | USP53 |
| UniProt Entry Name: | UBP53_HUMAN |
 | Western blot analysis of extracts of various cell lines, using USP53 antibody (CAB14353) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s. |
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