Cell Cycle Antibodies 1
Anti-USP44 Antibody (CAB15528)
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- SKU:
- CAB15528
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Description
| Antibody Name: | Anti-USP44 Antibody |
| Antibody SKU: | CAB15528 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 100-270 of human USP44 (NP_115523.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:200 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Jurkat, Raji, HT-29, Mouse lung, Mouse pancreas, Mouse liver, Rat stomach, Rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 100-270 of human USP44 (NP_115523.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | RTLS AIKS QNYH CTTR SGRF LRSM GTGD DSYF LHDG AQSL LQSE DQLY TALW HRRR ILMG KIFR TWFE QSPI GRKK QEEP FQEK IVVK REVK KRRQ ELEY QVKA ELES MPPR KSLR LQGL AQST IIEI VSVQ VPAQ TPAS PAKD KVLS TSEN EISQ KVSD SSVK RRPI VTP |
| Gene ID: | 84101 |
| Uniprot: | Q9H0E7 |
| Cellular Location: | Nucleus |
| Calculated MW: | 81kDa |
| Observed MW: | 81kDa |
| Synonyms: | USP44 |
| Background: | The protein encoded by this gene is a protease that functions as a deubiquitinating enzyme. The encoded protein is thought to help regulate the spindle assembly checkpoint by preventing early anaphase onset. This protein specifically deubiquitinates CDC20, which stabilizes the anaphase promoting complex/cyclosome. |
| UniProt Protein Function: | USP44: Deubiquitinase that plays a key role in the spindle checkpoint by preventing premature anaphase onset. Acts by specifically mediating deubiquitination of CDC20, a negative regulator of the anaphase promoting complex/cyclosome (APC/C). Deubiquitination of CDC20 leads to stabilize the MAD2L1-CDC20- APC/C ternary complex (also named mitotic checkpoint), thereby preventing premature activation of the APC/C. Belongs to the peptidase C19 family. USP44 subfamily. |
| UniProt Protein Details: | Protein type:Protease; EC 3.4.19.12; Ubiquitin-specific protease Chromosomal Location of Human Ortholog: 12q22 Cellular Component: microtubule cytoskeleton; nucleolus; nucleus Molecular Function:protein binding; ubiquitin-specific protease activity; zinc ion binding Biological Process: cell division; mitosis; proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination |
| NCBI Summary: | Modification of cellular proteins by ubiquitin is an essential regulatory mechanism controlled by the coordinated action of multiple ubiquitin-conjugating and deubiquitinating enzymes. USP44 belongs to a large family of cysteine proteases that function as deubiquitinating enzymes (Quesada et al., 2004 [PubMed 14715245]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | Q9H0E7 |
| NCBI GenInfo Identifier: | 300669621 |
| NCBI Gene ID: | 84101 |
| NCBI Accession: | Q9H0E7.2 |
| UniProt Secondary Accession: | Q9H0E7,B2RDW3, |
| UniProt Related Accession: | Q9H0E7 |
| Molecular Weight: | 81,185 Da |
| NCBI Full Name: | Ubiquitin carboxyl-terminal hydrolase 44 |
| NCBI Synonym Full Names: | ubiquitin specific peptidase 44 |
| NCBI Official Symbol: | USP44 |
| NCBI Protein Information: | ubiquitin carboxyl-terminal hydrolase 44 |
| UniProt Protein Name: | Ubiquitin carboxyl-terminal hydrolase 44 |
| UniProt Synonym Protein Names: | Deubiquitinating enzyme 44; Ubiquitin thioesterase 44; Ubiquitin-specific-processing protease 44 |
| Protein Family: | Ubiquitin carboxyl-terminal hydrolase |
| UniProt Gene Name: | USP44 |
| UniProt Entry Name: | UBP44_HUMAN |
 | Western blot analysis of extracts of various cell lines, using USP44 antibody (CAB15528) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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