Antibody Name:	Anti-USP36 Antibody
Antibody SKU:	CAB13115
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human USP36 (NP_079366.3).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	U-87MG, A-431, HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human USP36 (NP_079366.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MPIV DKLK EALK PGRK DSAD DGEL GKLL ASSA KKVL LQKI EFEP ASKS FSYQ LEAL KSKY VLLN PKTE GASR HKSG DDPP ARRQ GSEH TYES CGDG VPAP QKVL FPTE RLSL RWER VFRV
Gene ID:	57602
Uniprot:	Q9P275
Cellular Location:	Nucleus, nucleolus
Calculated MW:	122kDa
Observed MW:	123kDa

Synonyms:

USP36, DUB1

Background:

This gene encodes a member of the peptidase C19 or ubiquitin-specific protease family of cysteine proteases. Members of this family remove ubiquitin molecules from polyubiquitinated proteins. The encoded protein may deubiquitinate and stabilize the transcription factor c-Myc, also known as MYC, an important oncoprotein known to be upregulated in most human cancers. The encoded protease may also regulate the activation of autophagy. This gene exhibits elevated expression in some breast and lung cancers.

UniProt Protein Function:	USP36: May be required for maintaining multiple types of adult stem cells. May function as a transcriptional repressor by continually deubiquiting histone H2B at the promoters of genes critical for cellular differentiation, thereby preventing histone H3 'Lys-4' trimethylation (H3K4). Belongs to the peptidase C19 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Ubiquitin-specific protease; Ubiquitin conjugating system; EC 3.4.19.12; Nucleolus; Protease Chromosomal Location of Human Ortholog: 17q25.3 Cellular Component: nucleolus; nucleus Molecular Function:ubiquitin-specific protease activity Biological Process: protein deubiquitination; ubiquitin-dependent protein catabolic process
NCBI Summary:	This gene encodes a member of the peptidase C19 or ubiquitin-specific protease family of cysteine proteases. Members of this family remove ubiquitin molecules from polyubiquitinated proteins. The encoded protein may deubiquitinate and stabilize the transcription factor c-Myc, also known as MYC, an important oncoprotein known to be upregulated in most human cancers. The encoded protease may also regulate the activation of autophagy. This gene exhibits elevated expression in some breast and lung cancers. [provided by RefSeq, Mar 2016]
UniProt Code:	Q9P275
NCBI GenInfo Identifier:	124056592
NCBI Gene ID:	57602
NCBI Accession:	Q9P275.3
UniProt Secondary Accession:	Q9P275,Q05C98, Q05DD0, Q6IQ38, Q8NDM8, Q9NVC8,
UniProt Related Accession:	Q9P275
Molecular Weight:	122,908 Da
NCBI Full Name:	Ubiquitin carboxyl-terminal hydrolase 36
NCBI Synonym Full Names:	ubiquitin specific peptidase 36
NCBI Official Symbol:	USP36
NCBI Official Synonym Symbols:	DUB1
NCBI Protein Information:	ubiquitin carboxyl-terminal hydrolase 36
UniProt Protein Name:	Ubiquitin carboxyl-terminal hydrolase 36
UniProt Synonym Protein Names:	Deubiquitinating enzyme 36; Ubiquitin thioesterase 36; Ubiquitin-specific-processing protease 36
Protein Family:	Ubiquitin carboxyl-terminal hydrolase
UniProt Gene Name:	USP36
UniProt Entry Name:	UBP36_HUMAN

	Western blot analysis of extracts of various cell lines, using USP36 antibody (CAB13115) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
	Immunofluorescence analysis of HeLa cells using USP36 Polyclonal Antibody (CAB13115) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH-3T3 cells using USP36 Polyclonal Antibody (CAB13115) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.