KO Validated Antibodies 2

Anti-USP19 Antibody (CAB20006)[KO Validated]

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SKU:
CAB20006
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Signal Transduction

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-USP19 Antibody [KO Validated]
Antibody SKU:CAB20006
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant protein of human USP19.
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:293T
Immunogen:Recombinant protein of human USP19.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:10869
Uniprot:O94966
Cellular Location:Endoplasmic reticulum membrane, Single-pass membrane protein
Calculated MW:71kDa/140-156kDa
Observed MW:170KDa
Anti-USP19 Antibody [KO Validated] (CAB20006)Western blot analysis of extracts from normal (control) and USP19 knockout (KO) 293T cells, using USP19 antibody (CAB20006) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Synonyms:USP19, ZMYND9
Background:Protein ubiquitination controls many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction. This dynamic process, involving ubiquitin conjugating enzymes and deubiquitinating enzymes, adds and removes ubiquitin. Deubiquitinating enzymes are cysteine proteases that specifically cleave ubiquitin from ubiquitin-conjugated protein substrates. This protein is a ubiquitin protein ligase and plays a role in muscle wasting. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:USP19: Deubiquitinating enzyme that regulates the degradation of various proteins. Deubiquitinates and prevents proteasomal degradation of RNF123 which in turn stimulates CDKN1B ubiquitin- dependent degradation thereby playing a role in cell proliferation. Involved in decreased protein synthesis in atrophying skeletal muscle. Modulates transcription of major myofibrillar proteins. Also involved in turnover of endoplasmic- reticulum-associated degradation (ERAD) substrates. Regulates the stability of BIRC2/c-IAP1 and BIRC3/c-IAP2 by preventing their ubiquitination. Required for cells to mount an appropriate response to hypoxia and rescues HIF1A from degradation in a non- catalytic manner. Plays an important role in 17 beta-estradiol (E2)-inhibited myogenesis. Decreases the levels of ubiquitinated proteins during skeletal muscle formation and acts to repress myogenesis. Exhibits a preference towards 'Lys-63'-linked Ubiquitin chains. Belongs to the peptidase C19 family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, integral; Ubiquitin-specific protease; Protease; Ubiquitin conjugating system; EC 3.4.19.12

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: cytosol; endoplasmic reticulum membrane; integral to membrane

Molecular Function:Hsp90 protein binding; metal ion binding; protein binding; ubiquitin protein ligase binding; ubiquitin-specific protease activity

Biological Process: ER-associated protein catabolic process; negative regulation of skeletal muscle development; protein deubiquitination; protein stabilization; regulation of protein stability

UniProt Code:O94966
NCBI GenInfo Identifier:152031724
NCBI Gene ID:10869
NCBI Accession:O94966.2
UniProt Secondary Accession:O94966,Q3KQW4, Q641Q9, Q6NZY8, Q86XV9, A5PKX8, A6H8U2 B4DGT3, B4DTZ0, E7EN22, E7ETS0, E9PEG8,
UniProt Related Accession:O94966
Molecular Weight:150,659 Da
NCBI Full Name:Ubiquitin carboxyl-terminal hydrolase 19
NCBI Synonym Full Names:ubiquitin specific peptidase 19
NCBI Official Symbol:USP19  
NCBI Official Synonym Symbols:ZMYND9  
NCBI Protein Information:ubiquitin carboxyl-terminal hydrolase 19
UniProt Protein Name:Ubiquitin carboxyl-terminal hydrolase 19
UniProt Synonym Protein Names:Deubiquitinating enzyme 19; Ubiquitin thioesterase 19; Ubiquitin-specific-processing protease 19; Zinc finger MYND domain-containing protein 9
UniProt Gene Name:USP19  
UniProt Entry Name:UBP19_HUMAN
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