Signal Transduction Antibodies 2

Anti-USP18 Antibody (CAB16739)

(No reviews yet) Write a Review
SKU:
CAB16739
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-USP18 Antibody
Antibody SKU:CAB16739
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 200-300 of human USP18 (NP_059110.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HepG2, RAW264.7
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 200-300 of human USP18 (NP_059110.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:DVDS KPLK TLED ALHC FFQP RELS SKSK CFCE NCGK KTRG KQVL KLTH LPQT LTIH LMRF SIRN SQTR KICH SLYF PQSL DFSQ ILPM KRES CDAE EQSG G
Gene ID:11274
Uniprot:Q9UMW8
Cellular Location:
Calculated MW:43kDa
Observed MW:35kDa
Synonyms:USP18, ISG43, UBP43, PTORCH2
Background:The protein encoded by this gene belongs to the ubiquitin-specific proteases (UBP) family of enzymes that cleave ubiquitin from ubiquitinated protein substrates. It is highly expressed in liver and thymus, and is localized to the nucleus. This protein efficiently cleaves only ISG15 (a ubiquitin-like protein) fusions, and deletion of this gene in mice results in a massive increase of ISG15 conjugates in tissues, indicating that this protein is a major ISG15-specific protease. Mice lacking this gene are also hypersensitive to interferon, suggesting a function of this protein in downregulating interferon responses, independent of its isopeptidase activity towards ISG15.
UniProt Protein Function:USP18: Can efficiently cleave only ISG15 fusions including native ISG15 conjugates linked via isopeptide bonds. Necessary to maintain a critical cellular balance of ISG15-conjugated proteins in both healthy and stressed organisms. Belongs to the peptidase C19 family.
UniProt Protein Details:

Protein type:EC 3.1.2.-; EC 3.4.19.-; Protease; Ubiquitin-specific protease

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: cytosol; nucleus

Molecular Function:ISG15-specific protease activity; protein binding; ubiquitin-specific protease activity

NCBI Summary:The protein encoded by this gene belongs to the ubiquitin-specific proteases (UBP) family of enzymes that cleave ubiquitin from ubiquitinated protein substrates. It is highly expressed in liver and thymus, and is localized to the nucleus. This protein efficiently cleaves only ISG15 (a ubiquitin-like protein) fusions, and deletion of this gene in mice results in a massive increase of ISG15 conjugates in tissues, indicating that this protein is a major ISG15-specific protease. Mice lacking this gene are also hypersensitive to interferon, suggesting a function of this protein in downregulating interferon responses, independent of its isopeptidase activity towards ISG15. [provided by RefSeq, Sep 2011]
UniProt Code:Q9UMW8
NCBI GenInfo Identifier:10720335
NCBI Gene ID:11274
NCBI Accession:Q9UMW8.1
UniProt Secondary Accession:Q9UMW8,Q53Y90, Q6IAD9, Q9NY71,
UniProt Related Accession:Q9UMW8
Molecular Weight:41,352 Da
NCBI Full Name:Ubl carboxyl-terminal hydrolase 18
NCBI Synonym Full Names:ubiquitin specific peptidase 18
NCBI Official Symbol:USP18  
NCBI Official Synonym Symbols:ISG43; UBP43  
NCBI Protein Information:ubl carboxyl-terminal hydrolase 18
UniProt Protein Name:Ubl carboxyl-terminal hydrolase 18
UniProt Synonym Protein Names:43 kDa ISG15-specific protease; hUBP43; ISG15-specific-processing protease; Ubl thioesterase 18
Protein Family:Ubl carboxyl-terminal hydrolase
UniProt Gene Name:USP18  
Anti-USP18 Antibody (CAB16739)Western blot analysis of extracts of various cell lines, using USP18 antibody (CAB16739) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
Anti-USP18 Antibody (CAB16739)Immunofluorescence analysis of A431 cells using USP18 antibody (CAB16739) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-USP18 Antibody (CAB16739)Immunofluorescence analysis of C6 cells using USP18 antibody (CAB16739) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-USP18 Antibody (CAB16739)Immunofluorescence analysis of L929 cells using USP18 antibody (CAB16739) at dilution of 1:100. Blue: DAPI for nuclear staining.
View AllClose

0 Reviews

View AllClose

Related Products

Anti-USP18 Antibody (CAB20406)

Anti-USP18 Antibody (CAB20406)

OverviewProduct Name:USP18 Rabbit pAbProduct Code:CAB20406Size:50uL, 100uLSynonyms:UBP4, UBP43, Ubp15, AW047653, 1110058H21Rik