Name: Anti-USP16 Antibody (CAB5861)
Brand: Cell Cycle Antibodies 1
SKU: CAB5861
Availability: OutOfStock

Antibody Name:	Anti-USP16 Antibody
Antibody SKU:	CAB5861
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat, Monkey
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human USP16 (NP_001001992.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat, Monkey
Positive Samples:	COS-7, 293T, NIH/3T3, Mouse brain, Mouse heart, Mouse spleen, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human USP16 (NP_001001992.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MGKK RTKG KTVP IDDS SETL EPVC RHIR KGLE QGNL KKAL VNVE WNIC QDCK TDNK VKDK AEEE TEEK PSVW LCLK CGHQ GCGR NSQE QHAL KHYL TPRS EPHC LVLS LDNW SVWC YVCD NEVQ YCSS NQLG QVVD YVRK QASI TTPK PEKD NGNI ELEN KKLE KESK NEQE REKK ENMA KENP PMNS PCQI TVKG LSNL GNTC FFNA VMQN LSQT PVLR ELLK EVKM SGTI VKIE PPDL ALTE PLEI NLEP PGPL TLAM SQFL NEMQ ETKK GVVT PKEL
Gene ID:	10600
Uniprot:	Q9Y5T5
Cellular Location:	Nucleus
Calculated MW:	46kDa/58kDa/91kDa/93kDa
Observed MW:	100kDa

Synonyms:

USP16, UBP-M, UBPM

Background:

This gene encodes a deubiquitinating enzyme that is phosphorylated at the onset of mitosis and then dephosphorylated at the metaphase/anaphase transition. It can deubiquitinate H2A, one of two major ubiquitinated proteins of chromatin, in vitro and a mutant form of the protein was shown to block cell division. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

UniProt Protein Function:	USP16: a ubiquitous ubiquitin-processing protease that may de-ubiquitinate one or more critical proteins that are involved in the condensation of mitotic chromosomes, possibly acting selectively on histones H2A and H2B, the major ubiquitinated proteins of chromatin. Is phosphorylated at the onset of mitosis and dephosphorylated during the metaphase/anaphase transition.
UniProt Protein Details:	Protein type:Protease; Ubiquitin conjugating system; EC 3.4.19.12; Ubiquitin-specific protease Chromosomal Location of Human Ortholog: 21q22.11 Cellular Component: cytoplasm; nucleus Molecular Function:cysteine-type endopeptidase activity; histone binding; transcription coactivator activity; ubiquitin binding; ubiquitin-specific protease activity; zinc ion binding Biological Process: cell cycle; histone deubiquitination; mitosis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of translational elongation; protein homotetramerization; regulation of transcription from RNA polymerase II promoter; response to DNA damage stimulus
NCBI Summary:	This gene encodes a deubiquitinating enzyme that is phosphorylated at the onset of mitosis and then dephosphorylated at the metaphase/anaphase transition. It can deubiquitinate H2A, one of two major ubiquitinated proteins of chromatin, in vitro and a mutant form of the protein was shown to block cell division. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9Y5T5
NCBI GenInfo Identifier:	6686071
NCBI Gene ID:	10600
NCBI Accession:	Q9Y5T5.1
UniProt Secondary Accession:	Q9Y5T5,Q53GP7, Q53HA0, Q5VKN8, Q8NEL3, Q9H3E6, A8MU43 B3KN13, B4DFV8, B4DY37, D3DSD9,
UniProt Related Accession:	Q9Y5T5
Molecular Weight:	46,599 Da
NCBI Full Name:	Ubiquitin carboxyl-terminal hydrolase 16
NCBI Synonym Full Names:	ubiquitin specific peptidase 16
NCBI Official Symbol:	USP16
NCBI Official Synonym Symbols:	UBPM; UBP-M
NCBI Protein Information:	ubiquitin carboxyl-terminal hydrolase 16
UniProt Protein Name:	Ubiquitin carboxyl-terminal hydrolase 16
UniProt Synonym Protein Names:	Deubiquitinating enzyme 16
UniProt Gene Name:	USP16
UniProt Entry Name:	UBP16_HUMAN

Western blot analysis of extracts of various cell lines, using USP16 antibody (CAB5861) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

USP16 Antibody (PACO02340)

USP16 Antibody (PACO13121)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)