Immunology Antibodies 2

Anti-USP15 Antibody (CAB6786)

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SKU:
CAB6786
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

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Antibody Name:Anti-USP15 Antibody
Antibody SKU:CAB6786
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 852-952 of human USP15 (NP_006304.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:Human, Mouse
Positive Samples:293T, LO2
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 852-952 of human USP15 (NP_006304.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SNHY GGMG GGHY TAFA KNKD DGKW YYFD DSSV STAS EDQI VSKA AYVL FYQR QDTF SGTG FFPL DRET KGAS AATG IPLE SDED SNDN DNDI ENEN CMHT N
Gene ID:9958
Uniprot:Q9Y4E8
Cellular Location:Cytoplasm, Nucleus
Calculated MW:27kDa/109kDa/112kDa
Observed MW:112kDa
Synonyms:USP15, UNPH-2, UNPH4
Background:This gene encodes a member of the ubiquitin specific protease (USP) family of deubiquitinating enzymes. USP enzymes play critical roles in ubiquitin-dependent processes through polyubiquitin chain disassembly and hydrolysis of ubiquitin-substrate bonds. The encoded protein associates with the COP9 signalosome, and also plays a role in transforming growth factor beta signalling through deubiquitination of receptor-activated SMAD transcription factors. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 2.
UniProt Protein Function:USP15: Protease that degrades 'Lys-48'-linked polyubiquitin chains. Protects target proteins against proteasomal degradation. Protects APC and human papillomavirus type 16 protein E6 against degradation via the ubiquitin proteasome pathway. Belongs to the peptidase C19 family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Ubiquitin-specific protease; Protease; EC 3.4.19.12; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 12q14

Cellular Component: cytoplasm; nucleus

Molecular Function:cysteine-type endopeptidase activity; identical protein binding; protein binding; SMAD binding; transforming growth factor beta receptor binding; ubiquitin-specific protease activity

Biological Process: BMP signaling pathway; protein deubiquitination; transforming growth factor beta receptor signaling pathway

NCBI Summary:This gene encodes a member of the ubiquitin specific protease (USP) family of deubiquitinating enzymes. USP enzymes play critical roles in ubiquitin-dependent processes through polyubiquitin chain disassembly and hydrolysis of ubiquitin-substrate bonds. The encoded protein associates with the COP9 signalosome, and also plays a role in transforming growth factor beta signalling through deubiquitination of receptor-activated SMAD transcription factors. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 2. [provided by RefSeq, Nov 2011]
UniProt Code:Q9Y4E8
NCBI GenInfo Identifier:28381406
NCBI Gene ID:9958
NCBI Accession:Q9Y4E8.3
UniProt Secondary Accession:Q9Y4E8,Q08AL5, Q9H8G9, Q9HCA6, Q9UNP0, Q9Y5B5,
UniProt Related Accession:Q9Y4E8
Molecular Weight:27,094 Da
NCBI Full Name:Ubiquitin carboxyl-terminal hydrolase 15
NCBI Synonym Full Names:ubiquitin specific peptidase 15
NCBI Official Symbol:USP15  
NCBI Official Synonym Symbols:UNPH4; UNPH-2  
NCBI Protein Information:ubiquitin carboxyl-terminal hydrolase 15
UniProt Protein Name:Ubiquitin carboxyl-terminal hydrolase 15
UniProt Synonym Protein Names:Deubiquitinating enzyme 15; Ubiquitin thioesterase 15; Ubiquitin-specific-processing protease 15; Unph-2; Unph4
UniProt Gene Name:USP15  
UniProt Entry Name:UBP15_HUMAN
Anti-USP15 Antibody (CAB6786)Western blot analysis of extracts of various cell lines, using USP15 antibody (CAB6786) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-USP15 Antibody (CAB6786)Immunofluorescence analysis of HeLa cells using USP15 Polyclonal Antibody (CAB6786) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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