Immunology Antibodies 2
Anti-USP14 Antibody (CAB16643)
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- SKU:
- CAB16643
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-USP14 Antibody |
| Antibody SKU: | CAB16643 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide of human USP14 |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | U-87MG, HeLa, Mouse liver, Mouse brain |
| Immunogen: | A synthetic peptide of human USP14 |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 9097 |
| Uniprot: | P54578 |
| Cellular Location: | |
| Calculated MW: | 52kDa/54kDa/56kDa |
| Observed MW: | 60kDa |
| Synonyms: | USP14, TGT |
| Background: | This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. |
| UniProt Protein Function: | USP14: Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Indispensable for synaptic development and function at neuromuscular junctions (NMJs). Homodimer (Potential). Associates with the 26S proteasome. Interacts with FANCC, CXCR4 and ERN1. Belongs to the peptidase C19 family. USP14/UBP6 subfamily. |
| UniProt Protein Details: | Protein type:EC 3.4.19.12; Ubiquitin conjugating system; Protease; Ubiquitin-specific protease Chromosomal Location of Human Ortholog: 18p11.32 Cellular Component: cell surface; cytoplasm; cytoplasmic membrane-bound vesicle; plasma membrane; proteasome complex; synapse Molecular Function:cysteine-type endopeptidase activity; endopeptidase inhibitor activity; protein binding; tRNA guanylyltransferase activity; ubiquitin-specific protease activity Biological Process: protein deubiquitination; regulation of chemotaxis; synaptic transmission; ubiquitin-dependent protein catabolic process |
| NCBI Summary: | This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P54578 |
| NCBI GenInfo Identifier: | 82880645 |
| NCBI Gene ID: | 9097 |
| NCBI Accession: | NP_001032411.1 |
| UniProt Secondary Accession: | P54578,Q53XY5, B7Z4N8, J3QRZ5, |
| UniProt Related Accession: | P54578 |
| Molecular Weight: | 60.1 kDa |
| NCBI Full Name: | ubiquitin carboxyl-terminal hydrolase 14 isoform b |
| NCBI Synonym Full Names: | ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase) |
| NCBI Official Symbol: | USP14 |
| NCBI Official Synonym Symbols: | TGT |
| NCBI Protein Information: | ubiquitin carboxyl-terminal hydrolase 14 |
| UniProt Protein Name: | Ubiquitin carboxyl-terminal hydrolase 14 |
| UniProt Synonym Protein Names: | Deubiquitinating enzyme 14; Ubiquitin thioesterase 14; Ubiquitin-specific-processing protease 14 |
| Protein Family: | Ubiquitin carboxyl-terminal hydrolase |
| UniProt Gene Name: | USP14 |
| UniProt Entry Name: | UBP14_HUMAN |
 | Western blot analysis of extracts of various cell lines, using USP14 antibody (CAB16643) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 2min. |
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