Cell Biology Antibodies 8

Anti-USO1 Antibody (CAB2796)

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SKU:
CAB2796
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-USO1 Antibody
Antibody SKU:CAB2796
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human
Host Species:Rabbit
Immunogen:A synthetic peptide of human USO1
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human
Positive Samples:22Rv1, BT-474, K-562, THP-1, 293T, A-549
Immunogen:A synthetic peptide of human USO1
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:8615
Uniprot:O60763
Cellular Location:Cytoplasm, Golgi apparatus membrane, Peripheral membrane protein, cytosol
Calculated MW:107kDa/109kDa
Observed MW:115kDa
Synonyms:USO1, P115, TAP, VDP
Background:The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:TAP: General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity. Belongs to the VDP/USO1/EDE1 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Vesicle

Chromosomal Location of Human Ortholog: 4q21.1

Cellular Component: Golgi membrane; Golgi apparatus; Golgi stack; membrane; endoplasmic reticulum; perinuclear region of cytoplasm; nucleolus; cytosol

Molecular Function:protein transporter activity

Biological Process: intracellular protein transport; ER to Golgi vesicle-mediated transport; Golgi vesicle docking; vesicle fusion with Golgi apparatus; transcytosis; mitotic cell cycle

NCBI Summary:The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]
UniProt Code:O60763
NCBI GenInfo Identifier:143743108
NCBI Gene ID:8615
NCBI Accession:O60763.2
UniProt Related Accession:O60763
Molecular Weight:
NCBI Full Name:General vesicular transport factor p115
NCBI Synonym Full Names:USO1 vesicle transport factor
NCBI Official Symbol:USO1  
NCBI Official Synonym Symbols:TAP; VDP; P115  
NCBI Protein Information:general vesicular transport factor p115
UniProt Protein Name:General vesicular transport factor p115
UniProt Synonym Protein Names:Protein USO1 homolog; Transcytosis-associated protein; TAP; Vesicle-docking protein
Protein Family:Tripeptidyl aminopeptidase
UniProt Gene Name:USO1  
UniProt Entry Name:USO1_HUMAN
Anti-USO1 Antibody (CAB2796)Western blot analysis of extracts of various cell lines, using USO1 antibody (CAB2796) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-USO1 Antibody (CAB2796)Immunohistochemistry of paraffin-embedded human tonsil using USO1 antibody (CAB2796) at dilution of 1:100 (40x lens).
Anti-USO1 Antibody (CAB2796)Immunohistochemistry of paraffin-embedded human lung cancer using USO1 antibody (CAB2796) at dilution of 1:100 (40x lens).
Anti-USO1 Antibody (CAB2796)Immunohistochemistry of paraffin-embedded human colon carcinoma using USO1 antibody (CAB2796) at dilution of 1:100 (40x lens).
Anti-USO1 Antibody (CAB2796)Immunofluorescence analysis of HeLa cells using USO1 antibody (CAB2796). Blue: DAPI for nuclear staining.
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