Epigenetics & Nuclear Signaling Antibodies 4
Anti-USF2 Antibody (CAB8158)
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- SKU:
- CAB8158
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | USF2 Rabbit Polyclonal Antibody |
| Antibody SKU: | CAB8158 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 101-200 of human USF2 (NP_003358.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 101-200 of human USF2 (NP_003358.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | VVST AAFA GGQQ AVTQ VGVD GAAQ RPGP AAAS VPPG PAAP FPLA VIQN PFSN GGSP AAEA VSGE ARFA YFPA SSVG DTTA VSVQ TTDQ SLQA GGQF YVMM |
| Gene ID: | 7392 |
| Uniprot: | Q15853 |
| Cellular Location: | Nucleus |
| Calculated MW: | 23kDa/30kDa/36kDa |
| Observed MW: | 40kDa |
| Synonyms: | USF2, FIP, bHLHb12 |
| Background: | This gene encodes a member of the basic helix-loop-helix leucine zipper family of transcription factors. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs and is involved in regulating multiple cellular processes. |
| UniProt Protein Function: | USF2: Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:DNA-binding Chromosomal Location of Human Ortholog: 19q13 Cellular Component: intracellular membrane-bound organelle; nucleoplasm; nucleus Molecular Function:bHLH transcription factor binding; protein binding; protein heterodimerization activity; protein homodimerization activity; RNA polymerase II transcription factor activity, enhancer binding; sequence-specific DNA binding; transcription factor activity Biological Process: late viral mRNA transcription; lipid homeostasis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter by glucose; regulation of transcription from RNA polymerase II promoter by glucose; transcription from RNA polymerase II promoter |
| NCBI Summary: | This gene encodes a member of the basic helix-loop-helix leucine zipper family of transcription factors. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs and is involved in regulating multiple cellular processes. [provided by RefSeq, Mar 2016] |
| UniProt Code: | Q15853 |
| NCBI GenInfo Identifier: | 2833271 |
| NCBI Gene ID: | 7392 |
| NCBI Accession: | Q15853.1 |
| UniProt Secondary Accession: | Q15853,O00671, O00709, Q05750, Q07952, Q15851, Q15852 Q6FI33, Q6YI47, |
| UniProt Related Accession: | Q15853 |
| Molecular Weight: | 23,988 Da |
| NCBI Full Name: | Upstream stimulatory factor 2 |
| NCBI Synonym Full Names: | upstream transcription factor 2, c-fos interacting |
| NCBI Official Symbol: | USF2 |
| NCBI Official Synonym Symbols: | FIP; bHLHb12 |
| NCBI Protein Information: | upstream stimulatory factor 2 |
| UniProt Protein Name: | Upstream stimulatory factor 2 |
| UniProt Synonym Protein Names: | Class B basic helix-loop-helix protein 12; bHLHb12; FOS-interacting protein; FIP; Major late transcription factor 2; Upstream transcription factor 2 |
| Protein Family: | Upstream stimulatory factor |
| UniProt Gene Name: | USF2 |
| UniProt Entry Name: | USF2_HUMAN |
 | Western blot analysis of extracts of Rat liver, using USF2 antibody (CAB8158) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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