Name: Anti-USE1 Antibody (CAB13151)
Brand: Cell Biology Antibodies 3
SKU: CAB13151
Availability: OutOfStock

Antibody Name:	Anti-USE1 Antibody
Antibody SKU:	CAB13151
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-259 of human USE1 (NP_060937.1).

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-259 of human USE1 (NP_060937.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAAS RLEL NLVR LLSR CEAM AAEK RDPD EWRL EKYV GALE DMLQ ALKV HASK PASE VINE YSWK VDFL KGML QAEK LTSS SEKA LANQ FLAP GRVP TTAR ERVP ATKT VHLQ SRAR YTSE MRSE LLGT DSAE PEMD VRKR TGVA GSQP VSEK QSAA ELDL VLQR HQNL QEKL AEEM LGLA RSLK TNTL AAQS VIKK DNQT LSHS LKMA DQNL EKLK TESE RLEQ HTQK SVNW LLWA MLII VCFI FISM ILFI RIMP KLK
Gene ID:	55850
Uniprot:	Q9NZ43
Cellular Location:	Endoplasmic reticulum membrane, Single-pass type IV membrane protein
Calculated MW:	15kDa/16kDa/29kDa
Observed MW:	29kDa

Synonyms:	USE1, D12, MDS032, P31, SLT1
Background:

UniProt Protein Function:	USE1L: SNARE that may be involved in targeting and fusion of Golgi-derived retrograde transport vesicles with the ER. Belongs to the USE1 family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Vesicle; Membrane protein, integral Chromosomal Location of Human Ortholog: 19p13.11 Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; lysosome Molecular Function:protein binding Biological Process: ER to Golgi vesicle-mediated transport; lysosomal transport; protein catabolic process; retrograde vesicle-mediated transport, Golgi to ER; secretion by cell
UniProt Code:	Q9NZ43
NCBI GenInfo Identifier:	296452858
NCBI Gene ID:	55850
NCBI Accession:	Q9NZ43.2
UniProt Secondary Accession:	Q9NZ43,Q8NCK1, Q9BRT4,
UniProt Related Accession:	Q9NZ43
Molecular Weight:	15,063 Da
NCBI Full Name:	Vesicle transport protein USE1
NCBI Synonym Full Names:	unconventional SNARE in the ER 1
NCBI Official Symbol:	USE1
NCBI Official Synonym Symbols:	D12; P31; SLT1; MDS032
NCBI Protein Information:	vesicle transport protein USE1
UniProt Protein Name:	Vesicle transport protein USE1
UniProt Synonym Protein Names:	Putative MAPK-activating protein PM26; USE1-like protein; p31
Protein Family:	Protein transport protein
UniProt Gene Name:	USE1
UniProt Entry Name:	USE1_HUMAN

	Western blot analysis of extracts of various cell lines, using USE1 antibody (CAB13151) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of NIH-3T3 cells using USE1 Polyclonal Antibody (CAB13151) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.