Epigenetics & Nuclear Signaling Antibodies 1

Anti-URM1 Antibody (CAB13817)

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SKU:
CAB13817

Description

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Antibody Name:Anti-URM1 Antibody
Antibody SKU:CAB13817
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-63 of human URM1 (NP_001252511.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:Mouse, Rat
Positive Samples:Rat eye
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-63 of human URM1 (NP_001252511.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAAP LSVE VEFG GGAE LLFD GIKK HRVT LPGQ EEPW DIRN LLIW IKKN LLKE RPEL FIQG DSV
Gene ID:81605
Uniprot:Q9BTM9
Cellular Location:Cytoplasm
Calculated MW:7kDa/11kDa/15kDa
Observed MW:11kDa
Synonyms:URM1, C9orf74
Background:
UniProt Protein Function:URM1: Acts as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by thiocarboxylated (-COSH) at its C-terminus by MOCS3. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. May also act as an ubiquitin-like protein that is covalently conjugated to other proteins; the relevance of such function is however unclear in vivo. Belongs to the URM1 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Transferase; Ubiquitin-like modifier

Chromosomal Location of Human Ortholog: 9q34.11

Cellular Component: cytosol

Molecular Function:protein binding; sulfurtransferase activity

Biological Process: tRNA modification; tRNA wobble uridine modification

UniProt Code:Q9BTM9
NCBI GenInfo Identifier:68565265
NCBI Gene ID:81605
NCBI Accession:Q9BTM9.1
UniProt Secondary Accession:Q9BTM9,Q5T4B4, B3KMH3, B4DV08,
UniProt Related Accession:Q9BTM9
Molecular Weight:7,143 Da
NCBI Full Name:Ubiquitin-related modifier 1
NCBI Synonym Full Names:ubiquitin related modifier 1
NCBI Official Symbol:URM1  
NCBI Official Synonym Symbols:C9orf74  
NCBI Protein Information:ubiquitin-related modifier 1
UniProt Protein Name:Ubiquitin-related modifier 1
Protein Family:Ubiquitin-related modifier
UniProt Gene Name:URM1  
UniProt Entry Name:URM1_HUMAN
Anti-URM1 Antibody (CAB13817)Western blot analysis of extracts of rat eye, using URM1 antibody (CAB13817) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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Additional Information

Product Type:
Antibody
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
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