Cell Biology Antibodies 3
Anti-UQCR10 Antibody (CAB12587)
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- SKU:
- CAB12587
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-UQCR10 Antibody |
| Antibody SKU: | CAB12587 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-63 of human UQCR10 (NP_037519.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 22Rv1, NCI-H460, Mouse kidney, Rat heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-63 of human UQCR10 (NP_037519.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAAA TLTS KLYS LLFR RTST FALT IIVG VMFF ERAF DQGA DAIY DHIN EGKL WKHI KHKY ENK |
| Gene ID: | 29796 |
| Uniprot: | Q9UDW1 |
| Cellular Location: | Mitochondrion inner membrane |
| Calculated MW: | 6kDa/7kDa |
| Observed MW: | 10kDa |
| Synonyms: | UQCR10, HSPC051, HSPC119, HSPC151, QCR9, UCCR7.2, UCRC |
| Background: |
| UniProt Protein Function: | UCRC iso3: This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. This subunit interacts with cytochrome c1. Belongs to the UQCR10/QCR9 family. The bc1 complex contains 11 subunits: 3 respiratory subunits (cytochrome b, cytochrome c1 and Rieske/UQCRFS1), 2 core proteins (UQCRC1/QCR1 and UQCRC2/QCR2) and 6 low-molecular weight proteins (UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and a cleavage product of Rieske/UQCRFS1). |
| UniProt Protein Details: | Protein type:Mitochondrial; Energy Metabolism - oxidative phosphorylation Chromosomal Location of Human Ortholog: 22q12.2 Cellular Component: mitochondrial inner membrane; mitochondrial respiratory chain complex III Molecular Function:ubiquinol-cytochrome-c reductase activity Biological Process: cellular metabolic process; mitochondrial electron transport, ubiquinol to cytochrome c |
| NCBI Summary: | UCRC is a subunit of mitochondrial complex III (ubiquinol-cytochrome c reductase; EC 1.10.2.2), which forms the middle segment of the respiratory chain of the inner mitochondrial membrane (Schagger et al., 1995 [PubMed 8592474]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | Q9UDW1 |
| NCBI GenInfo Identifier: | 9297078 |
| NCBI Gene ID: | 29796 |
| NCBI Accession: | Q9UDW1.3 |
| UniProt Secondary Accession: | Q9UDW1,Q9T2V6, B5MCM5, |
| UniProt Related Accession: | Q9UDW1 |
| Molecular Weight: | 63 |
| NCBI Full Name: | Cytochrome b-c1 complex subunit 9 |
| NCBI Synonym Full Names: | ubiquinol-cytochrome c reductase, complex III subunit X |
| NCBI Official Symbol: | UQCR10 |
| NCBI Official Synonym Symbols: | QCR9; UCRC; HSPC051; HSPC119; HSPC151; UCCR7.2 |
| NCBI Protein Information: | cytochrome b-c1 complex subunit 9; cytochrome C1, nonheme 7kDa protein; cytochrome c1 non-heme 7 kDa protein; ubiquinol-cytochrome c reductase complex (7.2 kD); ubiquinol-cytochrome c reductase complex 7.2 kDa protein; ubiquinol-cytochrome c reductase, complex III subunit X, 7.2kDa |
| UniProt Protein Name: | Cytochrome b-c1 complex subunit 9 |
| UniProt Synonym Protein Names: | Complex III subunit 9; Complex III subunit X; Cytochrome c1 non-heme 7 kDa protein; Ubiquinol-cytochrome c reductase complex 7.2 kDa protein |
| UniProt Gene Name: | UQCR10 |
| UniProt Entry Name: | QCR9_HUMAN |
 | Western blot analysis of extracts of various cell lines, using UQCR10 antibody (CAB12587) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded rat ovary using UQCR10 antibody (CAB12587) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat heart using UQCR10 antibody (CAB12587) at dilution of 1:100 (40x lens). |
 | Confocal immunofluorescence analysis of U2OS cells using UQCR10 Polyclonal Antibody (CAB12587) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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