Antibody Name:	Anti-UPF3A Antibody
Antibody SKU:	CAB15893
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 327-476 of human UPF3A (NP_075387.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 327-476 of human UPF3A (NP_075387.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	EGSL EEPQ ETSH SGSD KEHR DVER SQEQ ESEA QRYH VDDG RRHR AHHE PERL SRRS EDEQ RWGK GPGQ DRGK KGSQ DSGA PGEA MERL GRAQ RCDD SPAP RKER LANK DRPA LQLY DPGA RFRA RECG GNRR ICKA EGSG TGPE KREE AE
Gene ID:	65110
Uniprot:	Q9H1J1
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	17kDa/51kDa/54kDa
Observed MW:	55kDa

Synonyms:

UPF3A, HUPF3A, RENT3A, UPF3

Background:

This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome 13. Two splice variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	UPF3A: Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC) and serving as link between the EJC core and NMD machinery. Recruits UPF2 at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2- UPF3 surveillance complex (including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. However, UPF3A is shown to be only marginally active in NMD as compared to UPF3B. Binds spliced mRNA upstream of exon-exon junctions. In vitro, weakly stimulates translation. Belongs to the RENT3 family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 13q34 Cellular Component: nucleoplasm; intracellular membrane-bound organelle; cytoplasm; plasma membrane; cytosol; nucleus Molecular Function:protein binding; RNA binding; nucleotide binding; nucleocytoplasmic transporter activity Biological Process: mRNA transport; positive regulation of translation; mRNA catabolic process, nonsense-mediated decay; nucleocytoplasmic transport; gene expression
NCBI Summary:	This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome 13. Two splice variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9H1J1
NCBI GenInfo Identifier:	27881818
NCBI Gene ID:	65110
NCBI Accession:	AAH23569.1
UniProt Secondary Accession:	Q9H1J1,Q5T8C3, Q5T8C9, Q7Z6N3, Q86YK1, Q9BZI8, A2A366
UniProt Related Accession:	Q9H1J1
Molecular Weight:	17,112 Da
NCBI Full Name:	UPF3 regulator of nonsense transcripts homolog A (yeast)
NCBI Synonym Full Names:	UPF3 regulator of nonsense transcripts homolog A (yeast)
NCBI Official Symbol:	UPF3A
NCBI Official Synonym Symbols:	UPF3; HUPF3A; RENT3A
NCBI Protein Information:	regulator of nonsense transcripts 3A; hUpf3; nonsense mRNA reducing factor 3A; up-frameshift suppressor 3 homolog A
UniProt Protein Name:	Regulator of nonsense transcripts 3A
UniProt Synonym Protein Names:	Nonsense mRNA reducing factor 3A; Up-frameshift suppressor 3 homolog A; hUpf3
Protein Family:	Regulator of nonsense transcripts
UniProt Gene Name:	UPF3A
UniProt Entry Name:	REN3A_HUMAN

	Western blot analysis of extracts of mouse testis, using UPF3A antibody (CAB15893) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
	Immunofluorescence analysis of C6 cells using UPF3A antibody (CAB15893) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of HeLa cells using UPF3A antibody (CAB15893) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using UPF3A antibody (CAB15893) at dilution of 1:100. Blue: DAPI for nuclear staining.