Name: Anti-UNC5B Antibody (CAB14978)
Brand: Cardiovascular Antibodies
SKU: CAB14978
Availability: OutOfStock

Antibody Name:	Anti-UNC5B Antibody
Antibody SKU:	CAB14978
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 290-377 of human UNC5B (NP_734465.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, A-549, 293T, Mouse brain, Mouse lung, Mouse kidney, Rat brain, Rat lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 290-377 of human UNC5B (NP_734465.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	FQKT ACTT ICPV DGAW TEWS KWSA CSTE CAHW RSRE CMAP PPQN GGRD CSGT LLDS KNCT DGLC MQNK KTLS DPNS HLLE ASGD AALY
Gene ID:	219699
Uniprot:	Q8IZJ1
Cellular Location:	Cell membrane, Membrane raft, Single-pass type I membrane protein
Calculated MW:	102kDa/103kDa
Observed MW:	104kDa

Synonyms:

UNC5B, UNC5H2, p53RDL1

Background:

This gene encodes a member of the netrin family of receptors. This particular protein mediates the repulsive effect of netrin-1 and is a vascular netrin receptor. This encoded protein is also in a group of proteins called dependence receptors (DpRs) which are involved in pro- and anti-apoptotic processes. Many DpRs are involved in embryogenesis and in cancer progression. Two alternatively spliced transcript variants have been described for this gene.

UniProt Protein Function:	UNC5B: Receptor for netrin required for axon guidance. Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. Mediates apoptosis by activating DAPK1. In the absence of NTN1, activates DAPK1 by reducing its autoinhibitory phosphorylation at Ser-308 thereby increasing its catalytic activity. Belongs to the unc-5 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, integral; Receptor, misc. Chromosomal Location of Human Ortholog: 10q22.1 Molecular Function:protein binding Biological Process: negative regulation of neuron apoptosis; positive regulation of phosphoinositide 3-kinase cascade
NCBI Summary:	This gene encodes a member of the netrin family of receptors. This particular protein mediates the repulsive effect of netrin-1 and is a vascular netrin receptor. This encoded protein is also in a group of proteins called dependence receptors (DpRs) which are involved in pro- and anti-apoptotic processes. Many DpRs are involved in embryogenesis and in cancer progression. Two alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Oct 2011]
UniProt Code:	Q8IZJ1
NCBI GenInfo Identifier:	54036589
NCBI Gene ID:	219699
NCBI Accession:	Q8IZJ1.2
UniProt Secondary Accession:	Q8IZJ1,Q5T3R9, Q5T3S0, Q86SN3, Q8N1Y2, Q9H9F3,
UniProt Related Accession:	Q8IZJ1
Molecular Weight:	102,434 Da
NCBI Full Name:	Netrin receptor UNC5B
NCBI Synonym Full Names:	unc-5 netrin receptor B
NCBI Official Symbol:	UNC5B
NCBI Official Synonym Symbols:	UNC5H2; p53RDL1
NCBI Protein Information:	netrin receptor UNC5B
UniProt Protein Name:	Netrin receptor UNC5B
UniProt Synonym Protein Names:	Protein unc-5 homolog 2; Protein unc-5 homolog B; p53-regulated receptor for death and life protein 1
Protein Family:	Netrin receptor
UniProt Gene Name:	UNC5B
UniProt Entry Name:	UNC5B_HUMAN

Western blot analysis of extracts of various cell lines, using UNC5B antibody (CAB14978) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.

ELISA Kits

Human C5b / Complement Fragment 5b ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)