Cell Biology Antibodies 4

Anti-Uhrf2 Antibody (CAB13647)

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SKU:
CAB13647
Product Type:
Antibody
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-Uhrf2 Antibody
Antibody SKU:CAB13647
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 76-359 of mouse Uhrf2 (NP_659122.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse, Rat
Positive Samples:Mouse thymus, Mouse testis, Mouse liver, Rat thymus
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 76-359 of mouse Uhrf2 (NP_659122.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:DSSL PSTS KQND AQVK PSSH NPPK VKKT ARGG SSSQ PSTS ARTC LIDP GFGL YKVN ELVD ARDV GLGA WFEA HIHS VTRA SDGH SRGK TPLK NGSS YKRT NGNV NHNS KENT NKLD NVPS TSNS DSVA ADED VIYH IEYD EYPE SGIL EMNV KDLR PRAR TILK WNEL NVGD VVMV NYNV ENPG KRGF WYDA EITT LKTI SRTK KEVR VKVF LGGS EGTL NDCR VMSV DEIF KIEK PGAH PISF ADGK FLRK NDPE CDLC GGDP DKTC HMCS CHKC GEKR DPNM
Gene ID:109113
Uniprot:Q7TMI3
Cellular Location:Nucleus
Calculated MW:24kDa/57kDa/90kDa
Observed MW:90kDa
Synonyms:UHRF2, NIRF, RNF107, TDRD23, URF2, Uhrf2
Background:This gene encodes a nuclear protein which is involved in cell-cycle regulation. The encoded protein is a ubiquitin-ligase capable of ubiquinating PCNP (PEST-containing nuclear protein), and together they may play a role in tumorigenesis. The encoded protein contains an NIRF_N domain, a PHD finger, a set- and ring-associated (SRA) domain, and a RING finger domain and several of these domains have been shown to be essential for the regulation of cell proliferation. This protein may also have a role in intranuclear degradation of polyglutamine aggregates. Alternative splicing results in multiple transcript variants some of which are non-protein coding.
UniProt Protein Function:UHRF2: E3 ubiquitin-protein ligase that is an intermolecular hub protein in the cell cycle network. Through cooperative DNA and histone binding, may contribute to a tighter epigenetic control of gene expression in differentiated cells. Ubiquitinates cyclins, CCND1 and CCNE1, in an apparently phosphorylation-independent manner and induces G1 arrest. Also ubiquitinates PCNP leading to its degradation by the proteasome. Appears to contribute to tumorigenesis. Associated with various cancers. DNA copy number loss is found in multiple kinds of malignancies originating from the brain, breast, stomach, kidney, hematopoietic tissue and lung. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Ubiquitin ligase; EC 6.3.2.-; EC 6.3.2.19; Ubiquitin conjugating system; Ligase; Cell cycle regulation

Cellular Component: nuclear heterochromatin; nucleoplasm; nucleus

Molecular Function:histone binding; ubiquitin-protein ligase activity

Biological Process: cell differentiation; cell proliferation; maintenance of DNA methylation; protein autoubiquitination; protein ubiquitination; ubiquitin-dependent protein catabolic process

UniProt Code:Q7TMI3
NCBI GenInfo Identifier:67462063
NCBI Gene ID:109113
NCBI Accession:Q7TMI3.1
UniProt Secondary Accession:Q7TMI3,Q8BG56, Q8BJP6, Q8BY30, Q8K1I5,
UniProt Related Accession:Q7TMI3
Molecular Weight:24,138 Da
NCBI Full Name:E3 ubiquitin-protein ligase UHRF2
NCBI Synonym Full Names:ubiquitin-like, containing PHD and RING finger domains 2
NCBI Official Symbol:Uhrf2  
NCBI Official Synonym Symbols:Nirf; AI426270; AW214556; 2310065A22Rik; D130071B19Rik  
NCBI Protein Information:E3 ubiquitin-protein ligase UHRF2
UniProt Protein Name:E3 ubiquitin-protein ligase UHRF2
UniProt Synonym Protein Names:NIRF; Np95-like ring finger protein; Nuclear protein 97; Nuclear zinc finger protein Np97; Ubiquitin-like PHD and RING finger domain-containing protein 2; Ubiquitin-like-containing PHD and RING finger domains protein 2
Protein Family:E3 ubiquitin-protein ligase
UniProt Gene Name:Uhrf2  
UniProt Entry Name:UHRF2_MOUSE
Anti-Uhrf2 Antibody (CAB13647)Western blot analysis of extracts of various cell lines, using Uhrf2 antibody (CAB13647) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
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