Name: Anti-UGT8 Antibody (CAB16442)
Brand: Metabolism Antibodies 2
SKU: CAB16442
Availability: OutOfStock

Antibody Name:	Anti-UGT8 Antibody
Antibody SKU:	CAB16442
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 380-460 of human UGT8 (NP_003351.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:	K-562

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 380-460 of human UGT8 (NP_003351.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	FGDH YDTM TRVQ AKGM GILL EWKT VTEK ELYE ALVK VINN PSYR QRAQ KLSE IHKD QPGH PVNR TIYW IDYI IRHN GAHH L
Gene ID:	7368
Uniprot:	Q16880
Cellular Location:	Membrane, Single-pass membrane protein
Calculated MW:	61kDa
Observed MW:	71kDa

Synonyms:	UGT8, CGT, UGT4
Background:	The protein encoded by this gene belongs to the UDP-glycosyltransferase family. It catalyzes the transfer of galactose to ceramide, a key enzymatic step in the biosynthesis of galactocerebrosides, which are abundant sphingolipids of the myelin membrane of the central and peripheral nervous systems. Alternatively spliced transcript variants have been found for this gene.

UniProt Protein Function:	UGT8: Catalyzes the transfer of galactose to ceramide, a key enzymatic step in the biosynthesis of galactocerebrosides, which are abundant sphingolipids of the myelin membrane of the central nervous system and peripheral nervous system. Belongs to the UDP-glycosyltransferase family.
UniProt Protein Details:	Protein type:Transferase; Lipid Metabolism - sphingolipid; EC 2.4.1.45; Membrane protein, integral Chromosomal Location of Human Ortholog: 4q26 Cellular Component: intracellular membrane-bound organelle; integral to membrane Molecular Function:UDP-galactose:glucosylceramide beta-1,4-galactosyltransferase activity; glucuronosyltransferase activity; 2-hydroxyacylsphingosine 1-beta-galactosyltransferase activity Biological Process: central nervous system development; flavonoid biosynthetic process; galactosylceramide biosynthetic process; axon cargo transport; cytoskeleton organization and biogenesis; neurite morphogenesis; cellular response to hormone stimulus; peripheral nervous system development; paranodal junction assembly
NCBI Summary:	The protein encoded by this gene belongs to the UDP-glycosyltransferase family. It catalyzes the transfer of galactose to ceramide, a key enzymatic step in the biosynthesis of galactocerebrosides, which are abundant sphingolipids of the myelin membrane of the central and peripheral nervous systems. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2011]
UniProt Code:	Q16880
NCBI GenInfo Identifier:	189491660
NCBI Gene ID:	7368
NCBI Accession:	NP_001121646.1
UniProt Secondary Accession:	Q16880,O00196, B3KXU7,
UniProt Related Accession:	Q16880
Molecular Weight:	61,438 Da
NCBI Full Name:	2-hydroxyacylsphingosine 1-beta-galactosyltransferase
NCBI Synonym Full Names:	UDP glycosyltransferase 8
NCBI Official Symbol:	UGT8
NCBI Official Synonym Symbols:	CGT; UGT4
NCBI Protein Information:	2-hydroxyacylsphingosine 1-beta-galactosyltransferase; UDP-galactose ceramide galactosyltransferase; UDP-galactose-ceramide galactosyltransferase; ceramide UDP-galactosyltransferase; cerebroside synthase; uridine diphosphate glycosyltransferase 8
UniProt Protein Name:	2-hydroxyacylsphingosine 1-beta-galactosyltransferase
UniProt Synonym Protein Names:	Ceramide UDP-galactosyltransferase; Cerebroside synthase; UDP-galactose-ceramide galactosyltransferase
Protein Family:	2-hydroxyacylsphingosine 1-beta-galactosyltransferase
UniProt Gene Name:	UGT8
UniProt Entry Name:	CGT_HUMAN

Western blot analysis of extracts of K-562 cells, using UGT8 antibody (CAB16442) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

Antibodies

UGT8 Antibody (PACO13085)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)