Cell Biology Antibodies 6

Anti-UGT1A4 Antibody (CAB15849)

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SKU:
CAB15849
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-UGT1A4 Antibody
Antibody SKU:CAB15849
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human UGT1A4 (NP_009051.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse liver, Mouse stomach, Rat liver
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human UGT1A4 (NP_009051.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MARG LQVP LPRL ATGL LLLL SVQP WAES GKVL VVPT DGSP WLSM REAL RELH ARGH QAVV LTPE VNMH IKEE KFFT LTAY AVPW TQKE FDRV TLGY TQGF
Gene ID:54657
Uniprot:P22310
Cellular Location:Endoplasmic reticulum membrane, Microsome, Single-pass membrane protein
Calculated MW:49kDa/60kDa
Observed MW:60kDa
Synonyms:UGT1A4, HUG-BR2, UDPGT, UDPGT 1-4, UGT-1D, UGT1-04, UGT1.4, UGT1A4S, UGT1D, UDPGT1-4
Background:This gene encodes a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5' exons may be spliced to the four common exons, resulting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site, and is regulated by its own promoter. This enzyme has some glucuronidase activity towards bilirubin, although is is more active on amines, steroids, and sapogenins.
UniProt Protein Function:UGT1A4: UDPGT is of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This isoform glucuronidates bilirubin IX- alpha to form both the IX-alpha-C8 and IX-alpha-C12 monoconjugates and diconjugate. Defects in UGT1A4 are the cause of Gilbert syndrome (GILBS). Gilbert syndrome occurs as a consequence of reduced bilirubin transferase activity and is often detected in young adults with vague nonspecific complaints. Defects in UGT1A4 are the cause of Crigler-Najjar syndrome type 1 (CN1). CN1 patients have severe hyperbilirubinemia and usually die of kernicterus (bilirubin accumulation in the basal ganglia and brainstem nuclei) within the first year of life. CN1 inheritance is autosomal recessive. Defects in UGT1A4 are the cause of Crigler-Najjar syndrome type 2 (CN2). CN2 patients have less severe hyperbilirubinemia and usually survive into adulthood without neurologic damage. Phenobarbital, which induces the partially deficient glucuronyl transferase, can diminish the jaundice. CN2 inheritance is autosomal dominant. Belongs to the UDP-glycosyltransferase family. 1 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Xenobiotic Metabolism - drug metabolism - other enzymes; Carbohydrate Metabolism - starch and sucrose; Endoplasmic reticulum; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; Lipid Metabolism - androgen and estrogen; Xenobiotic Metabolism - metabolism by cytochrome P450; EC 2.4.1.17; Transferase; Carbohydrate Metabolism - ascorbate and aldarate; Membrane protein, integral; Cofactor and Vitamin Metabolism - retinol; Carbohydrate Metabolism - pentose and glucuronate interconversions

Chromosomal Location of Human Ortholog: 2q37

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; integral to membrane

Molecular Function:protein homodimerization activity; enzyme binding; retinoic acid binding; glucuronosyltransferase activity; protein heterodimerization activity

Biological Process: negative regulation of transferase activity; flavonoid biosynthetic process; heme catabolic process; xenobiotic metabolic process; porphyrin metabolic process; negative regulation of fatty acid metabolic process; drug metabolic process; cellular response to hormone stimulus

Disease: Gilbert Syndrome; Crigler-najjar Syndrome, Type I

NCBI Summary:This gene encodes a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5' exons may be spliced to the four common exons, resulting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site, and is regulated by its own promoter. This enzyme has some glucuronidase activity towards bilirubin, although is is more active on amines, steroids, and sapogenins. [provided by RefSeq, Jul 2008]
UniProt Code:P22310
NCBI GenInfo Identifier:136731
NCBI Gene ID:54657
NCBI Accession:P22310.1
UniProt Secondary Accession:P22310,Q5DT00, B2R937, B8K288,
UniProt Related Accession:P22310
Molecular Weight:534
NCBI Full Name:UDP-glucuronosyltransferase 1-4
NCBI Synonym Full Names:UDP glucuronosyltransferase 1 family, polypeptide A4
NCBI Official Symbol:UGT1A4  
NCBI Official Synonym Symbols:UDPGT; UGT1D; UGT-1D; UGT1.4; HUG-BR2; UGT1-04; UDPGT 1-4  
NCBI Protein Information:UDP-glucuronosyltransferase 1-4; UDP-glucuronosyltransferase 1-D; UDP-glucuronosyltransferase 1A4; bilirubin-specific UDPGT isozyme 2; bilirubin UDP-glucuronosyltransferase isozyme 2; UDP glycosyltransferase 1 family, polypeptide A4
UniProt Protein Name:UDP-glucuronosyltransferase 1-4
UniProt Synonym Protein Names:Bilirubin-specific UDPGT isozyme 2; hUG-BR2; UDP-glucuronosyltransferase 1-D; UGT-1D; UGT1D; UDP-glucuronosyltransferase 1A4
Protein Family:UDP-glucuronosyltransferase
UniProt Gene Name:UGT1A4  
UniProt Entry Name:UD14_HUMAN
Anti-UGT1A4 Antibody (CAB15849)Western blot analysis of extracts of various cell lines, using UGT1A4 antibody (CAB15849) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
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