Description
Antibody Name: | Anti-UFM1 Antibody (CAB20875) |
Antibody SKU: | CAB20875 |
Antibody Size: | 50µL, 100µL |
Application: | Western blotting, Immunohistochemistry |
Reactivity: | Human, Mouse, Rat |
Host Species: | Rabbit |
Immunogen: | Recombinant protein of Human UFM1. |
Application: | Western blotting, Immunohistochemistry |
Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
Reactivity: | Human, Mouse, Rat |
Positive Samples: | HepG2, HL-60, SH-SY5Y, Mouse brain, Mouse spleen, Mouse pancreas, Rat brain, Rat spleen, Rat pancreas |
Immunogen: | Recombinant protein of Human UFM1. |
Purification Method: | Affinity purification |
Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
Isotype: | IgG |
Sequence: | Email for sequence |
Cellular Location: | Cytoplasm, Nucleus |
Calculated MW: | 9kDa/10kDa |
Observed MW: | 10KDa |
Synonyms: | UFM1, BM-002, C13orf20 |
Background: | UFM1 is a ubiquitin-like protein that is conjugated to target proteins by E1-like activating enzyme UBA5 (UBE1DC1; MIM 610552) and E2-like conjugating enzyme UFC1 (MIM 610554) in a manner analogous to ubiquitylation (see UBE2M; MIM 603173) (Komatsu et al., 2004 PubMed 15071506). |
Immunohistochemistry of paraffin-embedded human colon carcinoma using UFM1 Rabbit mAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. | |
Western blot analysis of extracts of various cell lines, using UFM1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s. |