Signal Transduction Antibodies 2
Anti-UFM1 Antibody (CAB15843)
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- SKU:
- CAB15843
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Description
| Antibody Name: | Anti-UFM1 Antibody |
| Antibody SKU: | CAB15843 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-85 of human UFM1 (NP_057701.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Mouse |
| Positive Samples: | Mouse pancreas |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-85 of human UFM1 (NP_057701.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSKV SFKI TLTS DPRL PYKV LSVP ESTP FTAV LKFA AEEF KVPA ATSA IITN DGIG INPA QTAG NVFL KHGS ELRI IPRD RVGS C |
| Gene ID: | 51569 |
| Uniprot: | P61960 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 9kDa/10kDa |
| Observed MW: | 11kDa |
| Synonyms: | UFM1, BM-002, C13orf20 |
| Background: | UFM1 is a ubiquitin-like protein that is conjugated to target proteins by E1-like activating enzyme UBA5 (UBE1DC1; MIM 610552) and E2-like conjugating enzyme UFC1 (MIM 610554) in a manner analogous to ubiquitylation (see UBE2M; MIM 603173) (Komatsu et al., 2004 [PubMed 15071506]). |
| UniProt Protein Function: | Ubiquitin-like modifier which can be covalently attached via an isopeptide bond to substrate proteins as a monomer or a lysine-linked polymer. The so-called ufmylation, requires the UFM1-activating E1 enzyme UBA5, the UFM1-conjugating E2 enzyme UFC1, and the UFM1-ligase E3 enzyme UFL1 (PubMed:15071506, PubMed:20018847). This post-translational modification on lysine residues of proteins may play a crucial role in a number of cellular processes. TRIP4 ufmylation may for instance play a role in nuclear receptors-mediated transcription (PubMed:25219498). Other substrates may include DDRGK1 with which it may play a role in the cellular response to endoplasmic reticulum stress (Probable). |
| NCBI Summary: | UFM1 is a ubiquitin-like protein that is conjugated to target proteins by E1-like activating enzyme UBA5 (UBE1DC1; MIM 610552) and E2-like conjugating enzyme UFC1 (MIM 610554) in a manner analogous to ubiquitylation (see UBE2M; MIM 603173) (Komatsu et al., 2004 [PubMed 15071506]).[supplied by OMIM, Dec 2008] |
| UniProt Code: | P61960 |
| NCBI GenInfo Identifier: | 7705300 |
| NCBI Gene ID: | 51569 |
| NCBI Accession: | NP_057701 |
| UniProt Secondary Accession: | P61960,Q14346, Q5VXS0, Q6IAG6, Q9CPX2, Q9NZF2, |
| UniProt Related Accession: | P61960 |
| Molecular Weight: | 11.1 kDa (103aa), confirmed by MALDI-TOF |
| NCBI Full Name: | ubiquitin-fold modifier 1 isoform 1 |
| NCBI Synonym Full Names: | ubiquitin fold modifier 1 |
| NCBI Official Symbol: | UFM1 |
| NCBI Official Synonym Symbols: | BM-002; C13orf20 |
| NCBI Protein Information: | ubiquitin-fold modifier 1 |
| UniProt Protein Name: | Ubiquitin-fold modifier 1 |
| Protein Family: | Ufm1-specific protease |
| UniProt Gene Name: | UFM1 |
 | Western blot analysis of extracts of mouse pancreas, using UFM1 antibody (CAB15843) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
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