Antibody Name:	Anti-UCN2 Antibody
Antibody SKU:	CAB6485
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-112 of human UCN2 (NP_149976.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Rat skin

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-112 of human UCN2 (NP_149976.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	VPVT PIPT FQLR PQNS PQTT PRPA ASES PSAA PTWP WAAQ SHCS PTRH PGSR IVLS LDVP IGLL QILL EQAR ARAA REQA TTNA RILA RVGH C
Gene ID:	90226
Uniprot:	Q96RP3
Cellular Location:	Secreted
Calculated MW:	12kDa
Observed MW:	12kDa

Synonyms:

UCN2, SRP, UCN-II, UCNI, UR, URP

Background:

This gene is a member of the sauvagine/corticotropin-releasing factor/urotensin I family. It is structurally related to the corticotropin-releasing factor (CRF) gene and the encoded product is an endogenous ligand for CRF type 2 receptors. In the brain it may be responsible for the effects of stress on appetite. In spite of the gene family name similarity, the product of this gene has no sequence similarity to urotensin II.

UniProt Protein Function:	UCN2: Suppresses food intake, delays gastric emptying and decreases heat-induced edema. Might represent an endogenous ligand for maintaining homeostasis after stress. Belongs to the sauvagine/corticotropin-releasing factor/urotensin I family.
UniProt Protein Details:	Protein type:Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 3p21.3 Cellular Component: extracellular space; extracellular region Molecular Function:hormone binding; G-protein-coupled receptor binding; hormone activity; corticotropin-releasing hormone receptor 2 binding; receptor binding Biological Process: cell proliferation; negative regulation of luteinizing hormone secretion; cellular response to nutrient levels; digestion; response to stress; cAMP biosynthetic process; G-protein signaling, adenylate cyclase activating pathway; negative regulation of follicle-stimulating hormone secretion
NCBI Summary:	This gene is a member of the sauvagine/corticotropin-releasing factor/urotensin I family. It is structurally related to the corticotropin-releasing factor (CRF) gene and the encoded product is an endogenous ligand for CRF type 2 receptors. In the brain it may be responsible for the effects of stress on appetite. In spite of the gene family name similarity, the product of this gene has no sequence similarity to urotensin II. [provided by RefSeq, Jul 2008]
UniProt Code:	Q96RP3
NCBI GenInfo Identifier:	20532289
NCBI Gene ID:	90226
NCBI Accession:	Q96RP3.1
UniProt Secondary Accession:	Q96RP3,Q9BUG0,
UniProt Related Accession:	Q96RP3
Molecular Weight:	Calculated: 12kDaObserved: Refer to figures
NCBI Full Name:	Urocortin-2
NCBI Synonym Full Names:	urocortin 2
NCBI Official Symbol:	UCN2
NCBI Official Synonym Symbols:	UR; SRP; URP; UCNI; UCN-II
NCBI Protein Information:	urocortin-2; ucn II; urocortin II; prepro-urocortin 2; urocortin-related peptide; stresscopin-related peptide
UniProt Protein Name:	Urocortin-2
UniProt Synonym Protein Names:	Stresscopin-related peptide; Urocortin II; Ucn II; Urocortin-related peptide
Protein Family:	Urocortin
UniProt Gene Name:	UCN2
UniProt Entry Name:	UCN2_HUMAN

	Western blot analysis of extracts of rat skin, using UCN2 antibody (CAB6485) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse heart using UCN2 antibody (CAB6485) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using UCN2 antibody (CAB6485). Blue: DAPI for nuclear staining.