Antibody Name:	Anti-UCKL1 Antibody
Antibody SKU:	CAB15851
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human UCKL1 (NP_060329.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse heart, Mouse lung, Rat pancreas

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human UCKL1 (NP_060329.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAAP PARA DADP SPTS PPTA RDTP GRQA EKSE TACE DRSN AESL DRLL PPVG TGRS PRKR TTSQ CKSE PPLL RTSK RTIY TAGR PPWY NEHG TQSK EAFA IGLG GGSA SGKT TVAR MIIE ALDV PWVV LLSM DSFY KVLT EQQQ EQAA HNNF NFDH PDAF DFDL IIST LKKL KQGK SVKV PIYD FTTH SRKK DWKT LYGA
Gene ID:	54963
Uniprot:	Q9NWZ5
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	44kDa/45kDa/59kDa/61kDa
Observed MW:	61kDa

Synonyms:

UCKL1, UCK1L, URKL1

Background:

The protein encoded by this gene is a uridine kinase. Uridine kinases catalyze the phosphorylation of uridine to uridine monophosphate. This protein has been shown to bind to Epstein-Barr nuclear antigen 3 as well as natural killer lytic-associated molecule. Ubiquitination of this protein is enhanced by the presence of natural killer lytic-associated molecule. In addition, protein levels decrease in the presence of natural killer lytic-associated molecule, suggesting that association with natural killer lytic-associated molecule results in ubiquitination and subsequent degradation of this protein. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	UCKL1: May contribute to UTP accumulation needed for blast transformation and proliferation. Belongs to the uridine kinase family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Nucleotide Metabolism - pyrimidine; EC 2.7.1.48; Xenobiotic Metabolism - drug metabolism - other enzymes; Transferase Chromosomal Location of Human Ortholog: 20q13.33 Cellular Component: cytoplasm; nucleus Molecular Function:protein binding; uridine kinase activity; ATP binding Biological Process: viral reproduction; phosphorylation
NCBI Summary:	The protein encoded by this gene is a uridine kinase. Uridine kinases catalyze the phosphorylation of uridine to uridine monophosphate. This protein has been shown to bind to Epstein-Barr nuclear antigen 3 as well as natural killer lytic-associated molecule. Ubiquitination of this protein is enhanced by the presence of natural killer lytic-associated molecule. In addition, protein levels decrease in the presence of natural killer lytic-associated molecule, suggesting that association with natural killer lytic-associated molecule results in ubiquitination and subsequent degradation of this protein. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]
UniProt Code:	Q9NWZ5
NCBI GenInfo Identifier:	84029407
NCBI Gene ID:	54963
NCBI Accession:	Q9NWZ5.2
UniProt Secondary Accession:	Q9NWZ5,Q5JWV0, Q70AQ5, Q8N524, Q9H3Z2, B7Z8N2,
UniProt Related Accession:	Q9NWZ5
Molecular Weight:	548
NCBI Full Name:	Uridine-cytidine kinase-like 1
NCBI Synonym Full Names:	uridine-cytidine kinase 1-like 1
NCBI Official Symbol:	UCKL1
NCBI Official Synonym Symbols:	UCK1L; URKL1
NCBI Protein Information:	uridine-cytidine kinase-like 1; UCK1-LIKE
UniProt Protein Name:	Uridine-cytidine kinase-like 1
Protein Family:	Uridine-cytidine kinase
UniProt Gene Name:	UCKL1
UniProt Entry Name:	UCKL1_HUMAN

	Western blot analysis of extracts of various cell lines, using UCKL1 antibody (CAB15851) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded Human breast cancer using UCKL1 Rabbit pAb (CAB15851) at dilution of 1:100 (40x lens).