Epigenetics & Nuclear Signaling Antibodies 4
Anti-UCHL5 Antibody (CAB7978)
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- SKU:
- CAB7978
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IF
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | UCHL5 Rabbit Polyclonal Antibody |
| Antibody SKU: | CAB7978 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-328 of human UCHL5 (NP_001186190.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | MCF7, HT-29, HeLa, NCI-H460, U-251MG, Mouse liver, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-328 of human UCHL5 (NP_001186190.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MTGN AGEW CLME SDPG VFTE LIKG FGCR GAQV EEIW SLEP ENFE KLKP VHGL IFLF KWQP GEEP AGSV VQDS RLDT IFFA KQVI NNAC ATQA IVSV LLNC THQD VHLG ETLS EFKE FSQS FDAA MKGL ALSN SDVI RQVH NSFA RQQM FEFD TKTS AKEE DAFH FVSY VPVN GRLY ELDG LREG PIDL GACN QDDW ISAV RPVI EKRI QKYS EGEI RFNL MAIV SDRK MIYE QKIA ELQR QLAE EPMD TDQG NSML SAIQ SEVA KNQM LIEE EVQK LKRY KIEN IRRK HNYL PFIM ELLK TLAE HQQL IPLV EKAK EKQN AKKA QETK |
| Gene ID: | 51377 |
| Uniprot: | Q9Y5K5 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 36kDa/37kDa |
| Observed MW: | 38kDa |
| Synonyms: | UCHL5, CGI-70, INO80R, UCH-L5, UCH37 |
| Background: |
| UniProt Code: | Q9Y5K5 |
| NCBI GenInfo Identifier: | 7706753 |
| NCBI Gene ID: | 51377 |
| NCBI Accession: | NP_057068 |
| UniProt Secondary Accession: | Q9Y5K5,Q5LJA6, Q5LJA7, Q8TBS4, Q96BJ9, Q9H1W5, Q9P0I3 Q9UQN2, |
| UniProt Related Accession: | Q9Y5K5 |
| Molecular Weight: | 39.7kDa (349aa), confirmed by MALDI-TOF |
| NCBI Full Name: | ubiquitin carboxyl-terminal hydrolase isozyme L5 isoform 1 |
| NCBI Synonym Full Names: | ubiquitin C-terminal hydrolase L5 |
| NCBI Official Symbol: | UCHL5 |
| NCBI Official Synonym Symbols: | UCH37; CGI-70; INO80R; UCH-L5 |
| NCBI Protein Information: | ubiquitin carboxyl-terminal hydrolase isozyme L5 |
| UniProt Protein Name: | Ubiquitin carboxyl-terminal hydrolase isozyme L5 |
| UniProt Synonym Protein Names: | Ubiquitin C-terminal hydrolase UCH37; Ubiquitin thioesterase L5 |
| UniProt Gene Name: | UCHL5 |
| UniProt Entry Name: | UCHL5_HUMAN |
 | Western blot analysis of extracts of various cell lines, using UCHL5 antibody (CAB7978) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 40s. |
 | Immunofluorescence analysis of A549 cells using UCHL5 antibody (CAB7978). Blue: DAPI for nuclear staining. |
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