Signal Transduction Antibodies 3

Anti-UCHL3 Antibody (CAB8156)

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SKU:
CAB8156
Product Type:
Antibody
Applications:
WB
Applications:
IHC
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Signal Transduction

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:UCHL3 Rabbit Polyclonal Antibody
Antibody SKU:CAB8156
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human UCHL3 (NP_005993.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:SH-SY5Y, HT-29, Mouse skeletal muscle, Mouse testis, Rat testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human UCHL3 (NP_005993.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MEGQ RWLP LEAN PEVT NQFL KQLG LHPN WQFV DVYG MDPE LLSM VPRP VCAV LLLF PITE KYEV FRTE EEEK IKSQ GQDV TSSV YFMK QTIS NACG TIGL IHAI ANNK DKMH FESG STLK KFLE ESVS MSPE ERAR YLEN YDAI RVTH ETSA HEGQ TEAP SIDE KVDL HFIA LVHV DGHL YELD GRKP FPIN HGET SDET LLED AIEV CKKF MERD PDEL
Gene ID:7347
Uniprot:P15374
Cellular Location:Cytoplasm
Calculated MW:26kDa
Observed MW:26kDa
Synonyms:UCHL3, UCH-L3
Background:The protein encoded by this gene is a member of the deubiquitinating enzyme family. Members of this family are proteases that catalyze the removal of ubiquitin from polypeptides and are divided into five classes, depending on the mechanism of catalysis. This protein may hydrolyze the ubiquitinyl-N-epsilon amide bond of ubiquitinated proteins to regenerate ubiquitin for another catalytic cycle. Alternative splicing results in multiple transcript variants that encode different protein isoforms.
UniProt Protein Function:UCHL3: Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3. Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin- signaling and insulin-induced adipogenesis. Required for stress- response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory. Can hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome and is associated with neurogenerative disorders. Preferentially binds diubiquitin; the interaction does not hydrolyze diubiquitin but, in vitro, inhibits the hydrolyzing activity on other substrates. Highly expressed in heart, skeletal muscle, and testis. Inhibited by monoubiquitin and diubiquitin. Belongs to the peptidase C12 family.
UniProt Protein Details:

Protein type:EC 3.4.19.12; Ubiquitin conjugating system; Protease

Chromosomal Location of Human Ortholog: 13q22.2

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function:peptidase activity; ubiquitin binding; protein binding; ubiquitin-specific protease activity

Biological Process: ubiquitin-dependent protein catabolic process; protein catabolic process

NCBI Summary:The protein encoded by this gene is a member of the deubiquitinating enzyme family. Members of this family are proteases that catalyze the removal of ubiquitin from polypeptides and are divided into five classes, depending on the mechanism of catalysis. This protein may hydrolyze the ubiquitinyl-N-epsilon amide bond of ubiquitinated proteins to regenerate ubiquitin for another catalytic cycle. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Aug 2012]
UniProt Code:P15374
NCBI GenInfo Identifier:5174741
NCBI Gene ID:7347
NCBI Accession:NP_005993.1
UniProt Secondary Accession:P15374,Q5TBK8, Q6IBE9, B2R970,
UniProt Related Accession:P15374
Molecular Weight:26,183 Da
NCBI Full Name:ubiquitin carboxyl-terminal hydrolase isozyme L3
NCBI Synonym Full Names:ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
NCBI Official Symbol:UCHL3  
NCBI Official Synonym Symbols:UCH-L3  
NCBI Protein Information:ubiquitin carboxyl-terminal hydrolase isozyme L3; OTTHUMP00000018509; ubiquitin thiolesterase; ubiquitin thioesterase L3
UniProt Protein Name:Ubiquitin carboxyl-terminal hydrolase isozyme L3
UniProt Synonym Protein Names:Ubiquitin thioesterase L3
Protein Family:Ubiquitin carboxyl-terminal hydrolase
UniProt Gene Name:UCHL3  
UniProt Entry Name:UCHL3_HUMAN
Anti-UCHL3 Polyclonal Antibody (CAB8156)Western blot analysis of extracts of various cell lines, using UCHL3 antibody (CAB8156) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-UCHL3 Polyclonal Antibody (CAB8156)Immunohistochemistry of paraffin-embedded human liver using UCHL3 antibody (CAB8156) at dilution of 1:100 (40x lens).
Anti-UCHL3 Polyclonal Antibody (CAB8156)Immunohistochemistry of paraffin-embedded human stomach using UCHL3 antibody (CAB8156) at dilution of 1:100 (40x lens).
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