Signal Transduction Antibodies 2

Anti-UCHL1 Antibody (CAB2131)

(No reviews yet) Write a Review
SKU:
CAB2131
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Signal Transduction

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-UCHL1 Antibody
Antibody SKU:CAB2131
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 59-223 of human UCHL1 (NP_004172.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:A-549, NCI-H460, Mouse brain, Mouse testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 59-223 of human UCHL1 (NP_004172.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:HENF RKKQ IEEL KGQE VSPK VYFM KQTI GNSC GTIG LIHA VANN QDKL GFED GSVL KQFL SETE KMSP EDRA KCFE KNEA IQAA HDAV AQEG QCRV DDKV NFHF ILFN NVDG HLYE LDGR MPFP VNHG ASSE DTLL KDAA KVCR EFTE REQG EVRF SAVA LCKA A
Gene ID:7345
Uniprot:P09936
Cellular Location:Cytoplasm, Endoplasmic reticulum membrane, Lipid-anchor
Calculated MW:24kDa
Observed MW:25kDa
Synonyms:HEL-117, HEL-S-53, NDGOA, PARK5, PGP 9.5, PGP9.5, PGP95, SPG79, Uch-L1, UCHL1
Background:The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.
UniProt Protein Function:UCHL1: Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. Monomer. Homodimer. Interacts with SNCA. Interacts with COPS5. Found in neuronal cell bodies and processes throughout the neocortex. Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients. Belongs to the peptidase C12 family.
UniProt Protein Details:

Protein type:Ubiquitin conjugating system; Cell development/differentiation; Ligase; Protease; EC 3.4.19.12

Chromosomal Location of Human Ortholog: 4p14

Cellular Component: nucleoplasm; endoplasmic reticulum membrane; cell soma; axon; cytoplasm; plasma membrane; cytosol

Molecular Function:ubiquitin binding; omega peptidase activity; protein binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity; alpha-2A adrenergic receptor binding; ligase activity

Biological Process: cell proliferation; proteasomal ubiquitin-dependent protein catabolic process; negative regulation of MAP kinase activity; axon transport of mitochondrion; protein deubiquitination; axon target recognition; eating behavior; neuromuscular process; muscle fiber development; sensory perception of pain; adult walking behavior

Disease: Neurodegeneration With Optic Atrophy, Childhood-onset; Parkinson Disease 5, Autosomal Dominant

NCBI Summary:The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.[provided by RefSeq, Sep 2009]
UniProt Code:P09936
NCBI GenInfo Identifier:136681
NCBI Gene ID:7345
NCBI Accession:P09936.2
UniProt Secondary Accession:P09936,Q4W5K6, Q71UM0,
UniProt Related Accession:P09936
Molecular Weight:223
NCBI Full Name:Ubiquitin carboxyl-terminal hydrolase isozyme L1
NCBI Synonym Full Names:ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
NCBI Official Symbol:UCHL1  
NCBI Official Synonym Symbols:NDGOA; PARK5; PGP95; PGP9.5; Uch-L1; HEL-117; PGP 9.5  
NCBI Protein Information:ubiquitin carboxyl-terminal hydrolase isozyme L1; ubiquitin thioesterase L1; epididymis luminal protein 117; neuron cytoplasmic protein 9.5; ubiquitin C-terminal hydrolase
UniProt Protein Name:Ubiquitin carboxyl-terminal hydrolase isozyme L1
UniProt Synonym Protein Names:Neuron cytoplasmic protein 9.5; PGP 9.5; PGP9.5; Ubiquitin thioesterase L1
Protein Family:Ubiquitin carboxyl-terminal hydrolase
UniProt Gene Name:UCHL1  
UniProt Entry Name:UCHL1_HUMAN
Anti-UCHL1 Antibody (CAB2131)Western blot analysis of extracts of various cell lines, using UCHL1 antibody (CAB2131) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Anti-UCHL1 Antibody (CAB2131)Immunofluorescence analysis of C6 cells using UCHL1 Rabbit pAb (CAB2131) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Anti-UCHL1 Antibody (CAB2131)Immunofluorescence analysis of U-251 MG cells using UCHL1 Rabbit pAb (CAB2131) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
View AllClose

0 Reviews

View AllClose

Related Products

Anti-PGP9.5/UCHL1 Antibody (CAB20380)

Anti-PGP9.5/UCHL1 Antibody (CAB20380)

OverviewAntibody Name:Anti-PGP9.5/UCHL1 Antibody (CAB20380)Antibody SKU:CAB20380Antibody Size:50µL, 100µLApplication:Western blotting, Immunohistochemist