Cell Biology Antibodies 4

Anti-UBLCP1 Antibody (CAB13729)

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SKU:
CAB13729
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-UBLCP1 Antibody
Antibody SKU:CAB13729
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 99-318 of human UBLCP1 (NP_659486.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:OVCAR3, A-549, SGC-7901, 293T, HeLa, Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 99-318 of human UBLCP1 (NP_659486.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:FDIE DEVV EVEN REEN LLKI SRRV KEYK VEIL NPPR EGKK LLVL DVDY TLFD HRSC AETG VELM RPYL HEFL TSAY EDYD IVIW SATN MKWI EAKM KELG VSTN ANYK ITFM LDSA AMIT VHTP RRGL IDVK PLGV IWGK FSEF YSKK NTIM FDDI GRNF LMNP QNGL KIRP FMKA HLNR DKDK ELLK LTQY LKEI AKLD DFLD LNHK YWER YLSK KQGQ
Gene ID:134510
Uniprot:Q8WVY7
Cellular Location:Nucleus
Calculated MW:36kDa
Observed MW:45kDa
Synonyms:UBLCP1, CPUB1
Background:
UniProt Protein Function:UBLCP1: Dephosphorylates 26S nuclear proteasomes, thereby decreasing their proteolytic activity. The dephosphorylation may prevent assembly of the core and regulatory particles (CP and RP) into mature 26S proteasome.
UniProt Protein Details:

Protein type:Hydrolase; EC 3.1.3.16

Chromosomal Location of Human Ortholog: 5q33.3

Cellular Component: nucleolus; nucleus

Molecular Function:protein binding; protein serine/threonine phosphatase activity

Biological Process: protein amino acid dephosphorylation

UniProt Code:Q8WVY7
NCBI GenInfo Identifier:74751564
NCBI Gene ID:134510
NCBI Accession:Q8WVY7.2
UniProt Secondary Accession:Q8WVY7,Q96DK5, D3DQJ7,
UniProt Related Accession:Q8WVY7
Molecular Weight:36,805 Da
NCBI Full Name:Ubiquitin-like domain-containing CTD phosphatase 1
NCBI Synonym Full Names:ubiquitin like domain containing CTD phosphatase 1
NCBI Official Symbol:UBLCP1  
NCBI Official Synonym Symbols:CPUB1  
NCBI Protein Information:ubiquitin-like domain-containing CTD phosphatase 1
UniProt Protein Name:Ubiquitin-like domain-containing CTD phosphatase 1
UniProt Synonym Protein Names:Nuclear proteasome inhibitor UBLCP1
Protein Family:Ubiquitin-like domain-containing CTD phosphatase
UniProt Gene Name:UBLCP1  
UniProt Entry Name:UBCP1_HUMAN
Anti-UBLCP1 Antibody (CAB13729)Western blot analysis of extracts of various cell lines, using UBLCP1 antibody (CAB13729) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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