Signal Transduction Antibodies 2
Anti-UBL4A Antibody (CAB4211)
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- SKU:
- CAB4211
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Signal Transduction
Description
| Antibody Name: | Anti-UBL4A Antibody |
| Antibody SKU: | CAB4211 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-157 of human UBL4A (NP_055050.1). |
| Application: | WB IP |
| Recommended Dilution: | WB 1:200 - 1:1000 IP 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, A-549, U-87MG, Mouse brain, Mouse kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-157 of human UBL4A (NP_055050.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MQLT VKAL QGRE CSLQ VPED ELVS TLKQ LVSE KLNV PVRQ QRLL FKGK ALAD GKRL SDYS IGPN SKLN LVVK PLEK VLLE EGEA QRLA DSPP PQVW QLIS KVLA RHFS AADA SRVL EQLQ RDYE RSLS RLTL DDIE RLAS RFLH PEVT ETME KGFS K |
| Gene ID: | 8266 |
| Uniprot: | P11441 |
| Cellular Location: | Cytoplasm, cytosol |
| Calculated MW: | 17kDa |
| Observed MW: | 14-16kDa |
| Synonyms: | UBL4A, DX254E, DXS254E, G6PD, GDX, GET5, MDY2, TMA24, UBL4 |
| Background: |
| UniProt Protein Function: | UBL4A: Component of the BAT3 complex, a multiprotein complex involved in the post-translational delivery of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane. TA membrane proteins, also named type II transmembrane proteins, contain a single C-terminal transmembrane region. The complex acts by facilitating TA proteins capture by ASNA1/TRC40: it is recruited to ribosomes synthesizing membrane proteins, interacts with the transmembrane region of newly released TA proteins, and transfers them to ASNA1/TRC40 for targeting. |
| UniProt Protein Details: | Protein type:Ubiquitin-like modifier Chromosomal Location of Human Ortholog: Xq28 Cellular Component: cytosol Molecular Function:protein binding; small conjugating protein ligase activity Biological Process: transport; protein modification process |
| UniProt Code: | P11441 |
| NCBI GenInfo Identifier: | 136662 |
| NCBI Gene ID: | 8266 |
| NCBI Accession: | P11441.1 |
| UniProt Secondary Accession: | P11441,Q5HY80, |
| UniProt Related Accession: | P11441 |
| Molecular Weight: | 157 |
| NCBI Full Name: | Ubiquitin-like protein 4A |
| NCBI Synonym Full Names: | ubiquitin-like 4A |
| NCBI Official Symbol: | UBL4A |
| NCBI Official Synonym Symbols: | GDX; G6PD; GET5; MDY2; UBL4; TMA24; DX254E; DXS254E |
| NCBI Protein Information: | ubiquitin-like protein 4A; ubiquitin-like 4; ubiquitin-like protein GDX |
| UniProt Protein Name: | Ubiquitin-like protein 4A |
| UniProt Synonym Protein Names: | Ubiquitin-like protein GDX |
| Protein Family: | Ubiquitin-like protein |
| UniProt Gene Name: | UBL4A |
| UniProt Entry Name: | UBL4A_HUMAN |
 | Western blot analysis of extracts of various cell lines, using UBL4A antibody (CAB4211) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug UBL4A antibody (CAB4211). Western blot was performed from the immunoprecipitate using UBL4A antibody (CAB4211) at a dilition of 1:1000. |
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