Name: Anti-UBIAD1 Antibody (CAB14214)
Brand: Metabolism Antibodies 1
SKU: CAB14214
Availability: OutOfStock

Antibody Name:	Anti-UBIAD1 Antibody
Antibody SKU:	CAB14214
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human UBIAD1 (NP_037451.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human UBIAD1 (NP_037451.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAAS QVLG EKIN ILSG ETVK AGDR DPLG NDCP EQDR LPQR SWRQ KCAS YVLA LRPW SFSA SLTP VALG SALA YRSH GVLD PRLL VGCA VAVL AVHG AGNL
Gene ID:	29914
Uniprot:	Q9Y5Z9
Cellular Location:	Cytoplasm, Endoplasmic reticulum membrane, Golgi apparatus membrane, Mitochondrion membrane, Multi-pass membrane protein, Nucleus
Calculated MW:	19kDa/36kDa
Observed MW:	90kDa

Synonyms:	UBIAD1, SCCD, TERE1
Background:	This gene encodes a protein thought to be involved in cholesterol and phospholipid metabolism. Mutations in this gene are associated with Schnyder crystalline corneal dystrophy.

UniProt Protein Function:	UBIAD1: Prenyltransferase that mediates the formation of menaquinone-4 (MK-4), a vitamin K2 isoform present at high concentrations in the brain, kidney and pancreas. Mediates the conversion of phylloquinone (PK) into menaquinone-4 (MK-4), probably by cleaving the side chain of phylloquinone (PK) to release 2-methyl-1,4-naphthoquinone (menadione; K3) and then prenylating it with geranylgeranyl pyrophosphate (GGPP) to form MK-4. Defects in UBIAD1 are the cause of crystalline corneal dystrophy of Schnyder (SCCD). SCCD is a rare autosomal dominant disease characterized by progressive corneal opacification resulting from abnormal deposition of cholesterol and phospholipids. Belongs to the UbiA prenyltransferase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 2.5.1.-; Membrane protein, integral; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 1p36.22 Cellular Component: cytoplasm; endoplasmic reticulum; endoplasmic reticulum membrane; integral to Golgi membrane; membrane; nucleus Molecular Function:antioxidant activity; prenyltransferase activity; protein binding Biological Process: menaquinone biosynthetic process; ubiquinone biosynthetic process; ubiquinone biosynthetic process via 3,4-dihydroxy-5-polyprenylbenzoate; vitamin K biosynthetic process; vitamin K metabolic process Disease: Schnyder Corneal Dystrophy
NCBI Summary:	This gene encodes a protein thought to be involved in cholesterol and phospholipid metabolism. Mutations in this gene are associated with Schnyder crystalline corneal dystrophy. [provided by RefSeq, Oct 2008]
UniProt Code:	Q9Y5Z9
NCBI GenInfo Identifier:	1057866085
NCBI Gene ID:	29914
NCBI Accession:
UniProt Secondary Accession:	Q9Y5Z9,Q53GX3, Q5THD4, B3KQG3,
UniProt Related Accession:	Q9Y5Z9
Molecular Weight:	Calculated MW 19kDa/36kDaObserved MW 37kDa
NCBI Full Name:	ubiA prenyltransferase domain-containing protein 1 isoform 2
NCBI Synonym Full Names:	UbiA prenyltransferase domain containing 1
NCBI Official Symbol:	UBIAD1
NCBI Official Synonym Symbols:	SCCD; TERE1
NCBI Protein Information:	ubiA prenyltransferase domain-containing protein 1
UniProt Protein Name:	UbiA prenyltransferase domain-containing protein 1
UniProt Synonym Protein Names:	Transitional epithelial response protein 1
Protein Family:	UbiA prenyltransferase domain-containing protein
UniProt Gene Name:	UBIAD1
UniProt Entry Name:	UBIA1_HUMAN

	Western blot analysis of extracts of HepG2 cells, using UBIAD1 Rabbit pAb (CAB14214) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of NIH/3T3 cells using UBIAD1 antibody (CAB14214) at dilution of 1:100. Blue: DAPI for nuclear staining.