Signal Transduction Antibodies 3

Anti-UBE2U Antibody (CAB8376)

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SKU:
CAB8376
Product Type:
Antibody
Applications:
WB
Applications:
IHC
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-UBE2U Antibody
Antibody SKU:CAB8376
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-226 of human UBE2U (NP_689702.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:Human, Mouse
Positive Samples:U-251MG, LO2, NCI-H460
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-226 of human UBE2U (NP_689702.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MHGR AYLL LHRD FCDL KENN YKGI TAKP VSED MMEW EVEI EGLQ NSVW QGLV FQLT IHFT SEYN YAPP VVKF ITIP FHPN VDPH TGQP CIDF LDNP EKWN TNYT LSSI LLAL QVML SNPV LENP VNLE AARI LVKD ESLY RTIL RLFN RPLQ MKDD SQEL PKDP RKCI RPIK TTSF SDYY QTWS RIAT SKAT EYYR TPLL KVPN FIGQ YYKW KKMD LQHQ KEWN LK
Gene ID:148581
Uniprot:Q5VVX9
Cellular Location:
Calculated MW:26kDa/37kDa
Observed MW:42kDa
Synonyms:UBE2U
Background:
UniProt Protein Function:UBE2U: Catalyzes the covalent attachment of ubiquitin to other proteins. Belongs to the ubiquitin-conjugating enzyme family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Ligase; EC 6.3.2.19; Ubiquitin ligase; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 1p31.3

Cellular Component: cytoplasm; nuclear chromatin

Molecular Function:protein binding; ubiquitin protein ligase binding

Biological Process: DNA repair; histone ubiquitination; proteasomal ubiquitin-dependent protein catabolic process; protein polyubiquitination

UniProt Code:Q5VVX9
NCBI GenInfo Identifier:74747311
NCBI Gene ID:148581
NCBI Accession:Q5VVX9.1
UniProt Secondary Accession:Q5VVX9,Q8N1D4,
UniProt Related Accession:Q5VVX9
Molecular Weight:26,689 Da
NCBI Full Name:Ubiquitin-conjugating enzyme E2 U
NCBI Synonym Full Names:ubiquitin conjugating enzyme E2 U (putative)
NCBI Official Symbol:UBE2U  
NCBI Protein Information:ubiquitin-conjugating enzyme E2 U
UniProt Protein Name:Ubiquitin-conjugating enzyme E2 U
UniProt Synonym Protein Names:E2 ubiquitin-conjugating enzyme U; Ubiquitin carrier protein U; Ubiquitin-protein ligase U
Protein Family:Ubiquitin-conjugating enzyme
UniProt Gene Name:UBE2U  
UniProt Entry Name:UBE2U_HUMAN
Anti-UBE2U Polyclonal Antibody (CAB8376)Western blot analysis of extracts of various cell lines, using UBE2U antibody (CAB8376) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-UBE2U Polyclonal Antibody (CAB8376)Immunohistochemistry of paraffin-embedded human liver using UBE2U antibody (CAB8376) at dilution of 1:100 (40x lens).
Anti-UBE2U Polyclonal Antibody (CAB8376)Immunohistochemistry of paraffin-embedded mouse brain using UBE2U antibody (CAB8376) at dilution of 1:100 (40x lens).
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