Epigenetics & Nuclear Signaling Antibodies 4

Anti-UBE2T Antibody (CAB6853)

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SKU:
CAB6853
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-UBE2T Antibody
Antibody SKU:CAB6853
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-197 of human UBE2T (NP_054895.1).
Application:WB
Recommended Dilution:WB 1:200 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse heart, Mouse thymus, Rat spleen, Rat heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-197 of human UBE2T (NP_054895.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MQRA SRLK RELH MLAT EPPP GITC WQDK DQMD DLRA QILG GANT PYEK GVFK LEVI IPER YPFE PPQI RFLT PIYH PNID SAGR ICLD VLKL PPKG AWRP SLNI ATVL TSIQ LLMS EPNP DDPL MADI SSEF KYNK PAFL KNAR QWTE KHAR QKQK ADEE EMLD NLPE AGDS RVHN STQK RKAS QLVG IEKK FHPD V
Gene ID:29089
Uniprot:Q9NPD8
Cellular Location:Nucleus
Calculated MW:22kDa
Observed MW:23kDa
Synonyms:UBE2T, FANCT, HSPC150, PIG50
Background:The protein encoded by this gene catalyzes the covalent attachment of ubiquitin to protein substrates. Defects in this gene have been associated with Fanconi anemia of complementation group T. Two transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:UBE2T: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in mitomycin-C (MMC)-induced DNA repair: acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. Also mediates monoubiquitination of FANCL and FANCI. May contribute to ubiquitination and degradation of BRCA1. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11'-, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Belongs to the ubiquitin-conjugating enzyme family.
UniProt Protein Details:

Protein type:Ubiquitin ligase; EC 6.3.2.19; Ligase; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function:small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; chromatin binding; ATP binding; ligase activity

Biological Process: protein monoubiquitination; protein autoubiquitination; DNA repair; response to DNA damage stimulus

Disease: Fanconi Anemia, Complementation Group T

NCBI Summary:The covalent conjugation of ubiquitin to proteins regulates diverse cellular pathways and proteins. Ubiquitin is transferred to a target protein through a concerted action of a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), such as UBE2T, and a ubiquitin ligase (E3) (Machida et al., 2006 [PubMed 16916645]).[supplied by OMIM, Mar 2008]
UniProt Code:Q9NPD8
NCBI GenInfo Identifier:73622065
NCBI Gene ID:29089
NCBI Accession:Q9NPD8.1
UniProt Secondary Accession:Q9NPD8,Q2TU36,
UniProt Related Accession:Q9NPD8
Molecular Weight:197
NCBI Full Name:Ubiquitin-conjugating enzyme E2 T
NCBI Synonym Full Names:ubiquitin-conjugating enzyme E2T
NCBI Official Symbol:UBE2T  
NCBI Official Synonym Symbols:PIG50; HSPC150  
NCBI Protein Information:ubiquitin-conjugating enzyme E2 T; ubiquitin-protein ligase T; ubiquitin carrier protein T; ubiquitin conjugating enzyme; cell proliferation-inducing gene 50 protein; ubiquitin-conjugating enzyme E2T (putative); HSPC150 protein similar to ubiquitin-conjugating enzyme
UniProt Protein Name:Ubiquitin-conjugating enzyme E2 T
UniProt Synonym Protein Names:Cell proliferation-inducing gene 50 protein; Ubiquitin carrier protein T; Ubiquitin-protein ligase T
Protein Family:Ubiquitin-conjugating enzyme
UniProt Gene Name:UBE2T  
UniProt Entry Name:UBE2T_HUMAN
Anti-UBE2T Antibody (CAB6853)Western blot analysis of extracts of various cell lines, using UBE2T antibody (CAB6853) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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