Name: Anti-UBE2S Antibody (CAB18064)[KO Validated]
Brand: KO Validated Antibodies 1
SKU: CAB18064
Availability: OutOfStock

Antibody Name:	Anti-UBE2S Antibody [KO Validated]
Antibody SKU:	CAB18064
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 122-221 of human UBE2S (NP_055316.2).

Application:	WB IHC
Recommended Dilution:	WB 1:200 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 122-221 of human UBE2S (NP_055316.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	HPNP ESAL NEEA GRLL LENY EEYA ARAR LLTE IHGG AGGP SGRA EAGR ALAS GTEA SSTD PGAP GGPG GAEG PMAK KHAG ERDK KLAA KKKT DKKR ALRR
Gene ID:	27338
Uniprot:	Q16763
Cellular Location:
Calculated MW:	23kDa
Observed MW:	26kDa

Anti-UBE2S Antibody [KO Validated] (CAB18064)

Western blot analysis of extracts from normal (control) and UBE2S knockout (KO) 293T cells, using UBE2S antibody (CAB18064) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.

Synonyms:	UBE2S, E2-EPF, E2EPF, EPF5
Background:	This gene encodes a member of the ubiquitin-conjugating enzyme family. The encoded protein is able to form a thiol ester linkage with ubiquitin in a ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins.

UniProt Protein Function:	UBE2S: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes 'Lys-11'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit. Also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it is however unclear whether UBE2D1/UBCH5 acts as a E2 enzyme for the APC/C in vivo. Also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, except 'Lys-48'-linked polyubiquitination. Component of the APC/C complex. Interacts with CDC20, FZR1/CDH1 and VHL. Belongs to the ubiquitin-conjugating enzyme family.
UniProt Protein Details:	Protein type:Ubiquitin ligase; EC 6.3.2.19; Ubiquitin conjugating system; Ligase Chromosomal Location of Human Ortholog: 19q13.43 Cellular Component: anaphase-promoting complex; cytoplasm Molecular Function:small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity Biological Process: anaphase-promoting complex activation; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell division; protein modification process; exit from mitosis
NCBI Summary:	This gene encodes a member of the ubiquitin-conjugating enzyme family. The encoded protein is able to form a thiol ester linkage with ubiquitin in a ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins. [provided by RefSeq, Jul 2008]
UniProt Code:	Q16763
NCBI GenInfo Identifier:	23829978
NCBI Gene ID:	27338
NCBI Accession:	Q16763.2
UniProt Related Accession:	Q16763
Molecular Weight:
NCBI Full Name:	Ubiquitin-conjugating enzyme E2 S
NCBI Synonym Full Names:	ubiquitin conjugating enzyme E2 S
NCBI Official Symbol:	UBE2S
NCBI Official Synonym Symbols:	EPF5; E2EPF; E2-EPF
NCBI Protein Information:	ubiquitin-conjugating enzyme E2 S
UniProt Protein Name:	Ubiquitin-conjugating enzyme E2 S
UniProt Synonym Protein Names:	E2-EPF; Ubiquitin carrier protein S; Ubiquitin-conjugating enzyme E2-24 kDa; Ubiquitin-conjugating enzyme E2-EPF5; Ubiquitin-protein ligase S
Protein Family:	Ubiquitin-conjugating enzyme
UniProt Gene Name:	UBE2S
UniProt Entry Name:	UBE2S_HUMAN