Name: Anti-UBE2O Antibody (CAB10036)[KO Validated]
Brand: KO Validated Antibodies 1
SKU: CAB10036
Availability: OutOfStock

Antibody Name:	Anti-UBE2O Antibody [KO Validated]
Antibody SKU:	CAB10036
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1073-1292 of human UBE2O (NP_071349.3).

Application:	WB IF IP
Recommended Dilution:	WB 1:1000 - 1:4000 IF 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-431, HepG2, Jurkat, Mouse heart, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1073-1292 of human UBE2O (NP_071349.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LVNE PYYN EAGF DSDR GLQE GYEN SRCY NEMA LIRV VQSM TQLV RRPP EVFE QEIR QHFS TGGW RLVN RIES WLET HALL EKAQ ALPN GVPK ASSS PEPP AVAE LSDS GQQE PEDG GPAP GEAS QGSD SEGG AQGL ASAS RDHT DQTS ETAP DASV PPSV KPKK RRKS YRSF LPEK SGYP DIGF PLFP LSKG FIKS IRGV LTQF RAAL LEAG MPEC TEDK
Gene ID:	63893
Uniprot:	Q9C0C9
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	141kDa
Observed MW:	200kDa

Synonyms:	UBE2O, E2-230K
Background:

UniProt Protein Function:	UBE2O: Catalyzes the covalent attachment of ubiquitin to other proteins. Belongs to the ubiquitin-conjugating enzyme family.
UniProt Protein Details:	Protein type:EC 6.3.2.19; Ubiquitin conjugating system; Ubiquitin ligase; Ligase Chromosomal Location of Human Ortholog: 17q25.1 Cellular Component: nucleoplasm; cytoplasm; nucleus Molecular Function:protein binding; small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity Biological Process: protein monoubiquitination; retrograde transport, endosome to Golgi; positive regulation of BMP signaling pathway
UniProt Code:	Q9C0C9
NCBI GenInfo Identifier:	192449449
NCBI Gene ID:	63893
NCBI Accession:	NP_071349.3
UniProt Secondary Accession:	Q9C0C9,Q69YP4, Q6PIZ2, Q86UA4, Q8N425, Q8TBN1, Q9BSW1 Q9H6E6, Q9H7E4, Q9H9B2, A6NDU5,
UniProt Related Accession:	Q9C0C9
Molecular Weight:
NCBI Full Name:	ubiquitin-conjugating enzyme E2 O
NCBI Synonym Full Names:	ubiquitin-conjugating enzyme E2O
NCBI Official Symbol:	UBE2O
NCBI Official Synonym Symbols:	E2-230K
NCBI Protein Information:	E2/E3 hybrid ubiquitin-protein ligase UBE2O; ubiquitin-protein ligase O; ubiquitin carrier protein O; ubiquitin-conjugating enzyme E2-230K; ubiquitin-conjugating enzyme E2 of 230 kDa
UniProt Protein Name:	E2/E3 hybrid ubiquitin-protein ligase UBE2O
UniProt Synonym Protein Names:	Ubiquitin carrier protein O; Ubiquitin-conjugating enzyme E2 O; Ubiquitin-conjugating enzyme E2 of 230 kDa; Ubiquitin-conjugating enzyme E2-230K; Ubiquitin-protein ligase O
Protein Family:	(E3-independent) E2 ubiquitin-conjugating enzyme
UniProt Gene Name:	UBE2O
UniProt Entry Name:	UBE2O_HUMAN

	Western blot analysis of extracts of various cell lines, using UBE2O antibody (CAB10036) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Western blot analysis of extracts from normal (control) and UBE2O knockout (KO) HeLa cells, using UBE2O antibody (CAB10036) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunofluorescence analysis of HeLa cells using UBE2O Polyclonal Antibody (CAB10036) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 200ug extracts of A-431 cells using 3ug UBE2O antibody (CAB10036). Western blot was performed from the immunoprecipitate using UBE2O antibody (CAB10036) at a dilition of 1:1000.