Cell Biology Antibodies 12

Anti-UBE2NL Antibody (CAB8381)

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SKU:
CAB8381
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-UBE2NL Antibody
Antibody SKU:CAB8381
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human UBE2NL (NP_001013007.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:HL-60, Mouse brain, Mouse testis, Mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human UBE2NL (NP_001013007.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAEL PHRI IKET QRLL AEPV PGIK AEPD ESNA RYFH VVIA GESK DSPF EGGT FKRE LLLA EEYP MAAP KVRF MTKI YHPN VDKL ERIS LDIL KDKW SPAL
Gene ID:389898
Uniprot:Q5JXB2
Cellular Location:
Calculated MW:17kDa
Observed MW:17kDa
Synonyms:UBE2NL, Li174
Background:
UniProt Protein Function:UBE2NL: This gene is intronless and encodes a member of the ubiquitin-conjugating enzyme family. The protein product is 91% identical to ubiquitin-conjugating enzyme E2N, a multi-exon gene product. [provided by RefSeq, Dec 2009]
UniProt Protein Details:

Protein type:EC 6.3.2.-; Ubiquitin ligase; Ligase

Chromosomal Location of Human Ortholog: Xq27.3

Cellular Component: cytoplasm; nucleus

Molecular Function:ubiquitin protein ligase binding

Biological Process: postreplication repair

NCBI Summary:This gene is intronless and encodes a member of the ubiquitin-conjugating enzyme family. The protein product is 91% identical to ubiquitin-conjugating enzyme E2N, a multi-exon gene product. This locus represents a polymorphic pseudogene, where some individuals contain an allele that can encode a full-length protein, while others have a non-functional allele containing a premature stop codon (reference SNP rs237520) that truncates the coding sequence. [provided by RefSeq, Jun 2014]
UniProt Code:Q5JXB2
NCBI GenInfo Identifier:74742728
NCBI Gene ID:389898
NCBI Accession:Q5JXB2.1
UniProt Secondary Accession:Q5JXB2,E9KL27,
UniProt Related Accession:Q5JXB2
Molecular Weight:17,377 Da
NCBI Full Name:Putative ubiquitin-conjugating enzyme E2 N-like
NCBI Synonym Full Names:ubiquitin conjugating enzyme E2 N like (gene/pseudogene)
NCBI Official Symbol:UBE2NL  
NCBI Official Synonym Symbols:Li174  
NCBI Protein Information:putative ubiquitin-conjugating enzyme E2 N-like
UniProt Protein Name:Putative ubiquitin-conjugating enzyme E2 N-like
UniProt Synonym Protein Names:Epididymis tissue protein Li 174
Protein Family:Putative ubiquitin-conjugating enzyme
UniProt Gene Name:UBE2NL  
UniProt Entry Name:UE2NL_HUMAN
Anti-UBE2NL Polyclonal Antibody (CAB8381)Western blot analysis of extracts of various cell lines, using UBE2NL antibody (CAB8381) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
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