Cell Biology Antibodies 12
Anti-UBE2NL Antibody (CAB8381)
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- SKU:
- CAB8381
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-UBE2NL Antibody |
| Antibody SKU: | CAB8381 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human UBE2NL (NP_001013007.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HL-60, Mouse brain, Mouse testis, Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human UBE2NL (NP_001013007.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAEL PHRI IKET QRLL AEPV PGIK AEPD ESNA RYFH VVIA GESK DSPF EGGT FKRE LLLA EEYP MAAP KVRF MTKI YHPN VDKL ERIS LDIL KDKW SPAL |
| Gene ID: | 389898 |
| Uniprot: | Q5JXB2 |
| Cellular Location: | |
| Calculated MW: | 17kDa |
| Observed MW: | 17kDa |
| Synonyms: | UBE2NL, Li174 |
| Background: |
| UniProt Protein Function: | UBE2NL: This gene is intronless and encodes a member of the ubiquitin-conjugating enzyme family. The protein product is 91% identical to ubiquitin-conjugating enzyme E2N, a multi-exon gene product. [provided by RefSeq, Dec 2009] |
| UniProt Protein Details: | Protein type:EC 6.3.2.-; Ubiquitin ligase; Ligase Chromosomal Location of Human Ortholog: Xq27.3 Cellular Component: cytoplasm; nucleus Molecular Function:ubiquitin protein ligase binding Biological Process: postreplication repair |
| NCBI Summary: | This gene is intronless and encodes a member of the ubiquitin-conjugating enzyme family. The protein product is 91% identical to ubiquitin-conjugating enzyme E2N, a multi-exon gene product. This locus represents a polymorphic pseudogene, where some individuals contain an allele that can encode a full-length protein, while others have a non-functional allele containing a premature stop codon (reference SNP rs237520) that truncates the coding sequence. [provided by RefSeq, Jun 2014] |
| UniProt Code: | Q5JXB2 |
| NCBI GenInfo Identifier: | 74742728 |
| NCBI Gene ID: | 389898 |
| NCBI Accession: | Q5JXB2.1 |
| UniProt Secondary Accession: | Q5JXB2,E9KL27, |
| UniProt Related Accession: | Q5JXB2 |
| Molecular Weight: | 17,377 Da |
| NCBI Full Name: | Putative ubiquitin-conjugating enzyme E2 N-like |
| NCBI Synonym Full Names: | ubiquitin conjugating enzyme E2 N like (gene/pseudogene) |
| NCBI Official Symbol: | UBE2NL |
| NCBI Official Synonym Symbols: | Li174 |
| NCBI Protein Information: | putative ubiquitin-conjugating enzyme E2 N-like |
| UniProt Protein Name: | Putative ubiquitin-conjugating enzyme E2 N-like |
| UniProt Synonym Protein Names: | Epididymis tissue protein Li 174 |
| Protein Family: | Putative ubiquitin-conjugating enzyme |
| UniProt Gene Name: | UBE2NL |
| UniProt Entry Name: | UE2NL_HUMAN |
 | Western blot analysis of extracts of various cell lines, using UBE2NL antibody (CAB8381) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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