Signal Transduction Antibodies 3

Anti-UBE2M Antibody (CAB9007)

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SKU:
CAB9007
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-UBE2M Antibody
Antibody SKU:CAB9007
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-183 of human UBE2M (NP_003960.1).
Application:WB
Recommended Dilution:WB 1:200 - 1:2000
Reactivity:Human, Mouse
Positive Samples:HeLa, 293T, Mouse kidney, Mouse heart, Mouse testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-183 of human UBE2M (NP_003960.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MIKL FSLK QQKK EEES AGGT KGSS KKAS AAQL RIQK DINE LNLP KTCD ISFS DPDD LLNF KLVI CPDE GFYK SGKF VFSF KVGQ GYPH DPPK VKCE TMVY HPNI DLEG NVCL NILR EDWK PVLT INSI IYGL QYLF LEPN PEDP LNKE AAEV LQNN RRLF EQNV QRSM RGGY IGST YFER CLK
Gene ID:9040
Uniprot:P61081
Cellular Location:
Calculated MW:20kDa
Observed MW:21kDa
Synonyms:UBE2M, UBC-RS2, UBC12, hUbc12
Background:The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin.
UniProt Protein Function:Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation.
NCBI Summary:The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin. [provided by RefSeq, Jul 2008]
UniProt Code:P61081
NCBI GenInfo Identifier:4507791
NCBI Gene ID:9040
NCBI Accession:NP_003960.1
UniProt Secondary Accession:P61081,O76069, Q8VC50,
UniProt Related Accession:P61081
Molecular Weight:Observed: 21 kDaPredicted: 21 kDa
NCBI Full Name:NEDD8-conjugating enzyme Ubc12
NCBI Synonym Full Names:ubiquitin conjugating enzyme E2 M
NCBI Official Symbol:UBE2M  
NCBI Official Synonym Symbols:UBC12; hUbc12; UBC-RS2  
NCBI Protein Information:NEDD8-conjugating enzyme Ubc12
UniProt Protein Name:NEDD8-conjugating enzyme Ubc12
UniProt Synonym Protein Names:NEDD8 carrier protein; Ubiquitin-conjugating enzyme E2 M
UniProt Gene Name:UBE2M  
Anti-UBE2M Polyclonal Antibody (CAB9007)Western blot analysis of extracts of various cell lines, using UBE2M antibody (CAB9007) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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