Anti-Ube2L3 / UBCH7 Antibody (CAB20887)
- SKU:
- CAB20887
- Product Type:
- Antibody
- Antibody Type:
- Polyclonal Antibody
- Reactivity:
- Plant
- Host Species:
- Rabbit
- Isotype:
- IgG
Description
| Antibody Name: | Anti-Ube2L3 / UBCH7 Antibody (CAB20887) |
| Antibody SKU: | CAB20887 |
| Antibody Size: | 50µL, 100µL |
| Application: | Western blotting, Immunohistochemistry, Immunofluorescence |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant protein of Human Ube2L3 / UBCH7. |
| Application: | Western blotting, Immunohistochemistry, Immunofluorescence |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, A-431, NIH/3T3, HL-60, K-562, Mouse testis, Mouse brain, Rat lung, Rat testis |
| Immunogen: | Recombinant protein of Human Ube2L3 / UBCH7. |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 14kDa/17kDa/24kDa |
| Observed MW: | 18KDa |
| Synonyms: | UBE2L3, E2-F1, L-UBC, UBCH7, UbcM4 |
| Background: | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Several alternatively spliced transcript variants have been found for this gene. |
 | Immunofluorescence analysis of U2OS cells using Ube2L3 / UBCH7 Rabbit mAb at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH/3T3 cells using Ube2L3 / UBCH7 Rabbit mAb at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of PC-12 cells using Ube2L3 / UBCH7 Rabbit mAb at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of K-562 cells using Ube2L3 / UBCH7 Rabbit mAb at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of HeLa cells using Ube2L3 / UBCH7 Rabbit mAb at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunohistochemistry of paraffin-embedded human placenta using Ube2L3 / UBCH7 Rabbit mAb at dilution of 1:25 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. |
 | Immunohistochemistry of paraffin-embedded human colon carcinoma using Ube2L3 / UBCH7 Rabbit mAb at dilution of 1:25 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. |
 | Western blot analysis of extracts of various cell lines, using Ube2L3/UBCH7 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s. |
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