Antibody Name:	Anti-UBE2J1 Antibody
Antibody SKU:	CAB15841
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human UBE2J1 (NP_057105.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse heart, Mouse kidney, Rat lung

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human UBE2J1 (NP_057105.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	METR YNLK SPAV KRLM KEAA ELKD PTDH YHAQ PLED NLFE WHFT VRGP PDSD FDGG VYHG RIVL PPEY PMKP PSII LLTA NGRF EVGK KICL SISG HHPE
Gene ID:	51465
Uniprot:	Q9Y385
Cellular Location:	Endoplasmic reticulum membrane, Single-pass type IV membrane protein
Calculated MW:	35kDa
Observed MW:	35kDa

Synonyms:

UBE2J1, CGI-76, HSPC153, HSPC205, HSU93243, NCUBE-1, NCUBE1, UBC6, UBC6E, Ubc6p

Background:

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum (ER) and may contribute to quality control ER-associated degradation by the ubiquitin-proteasome system.

UniProt Protein Function:	UBE2J1: Catalyzes the covalent attachment of ubiquitin to other proteins. Functions in the selective degradation of misfolded membrane proteins from the endoplasmic reticulum (ERAD). Belongs to the ubiquitin-conjugating enzyme family.
UniProt Protein Details:	Protein type:EC 6.3.2.19; Ligase; Membrane protein, integral; Ubiquitin conjugating system; Ubiquitin ligase Chromosomal Location of Human Ortholog: 6q15 Cellular Component: cytoplasm Molecular Function:ubiquitin protein ligase binding Biological Process: ER-associated protein catabolic process; protein amino acid N-linked glycosylation via asparagine
NCBI Summary:	The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum (ER) and may contribute to quality control ER-associated degradation by the ubiquitin-proteasome system. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9Y385
NCBI GenInfo Identifier:	52000881
NCBI Gene ID:	51465
NCBI Accession:	Q9Y385.2
UniProt Secondary Accession:	Q9Y385,Q53F25, Q5W0N4, Q9BZ32, Q9NQL3, Q9NY66, Q9P011 Q9P0S0, Q9UF10, A8K3F9,
UniProt Related Accession:	Q9Y385
Molecular Weight:	35,199 Da
NCBI Full Name:	Ubiquitin-conjugating enzyme E2 J1
NCBI Synonym Full Names:	ubiquitin conjugating enzyme E2 J1
NCBI Official Symbol:	UBE2J1
NCBI Official Synonym Symbols:	UBC6; UBC6E; Ubc6p; CGI-76; NCUBE1; HSPC153; HSPC205; NCUBE-1; HSU93243
NCBI Protein Information:	ubiquitin-conjugating enzyme E2 J1
UniProt Protein Name:	Ubiquitin-conjugating enzyme E2 J1
UniProt Synonym Protein Names:	E2 ubiquitin-conjugating enzyme J1; Non-canonical ubiquitin-conjugating enzyme 1; NCUBE-1; Yeast ubiquitin-conjugating enzyme UBC6 homolog E; HsUBC6e
Protein Family:	Ubiquitin-conjugating enzyme
UniProt Gene Name:	UBE2J1
UniProt Entry Name:	UB2J1_HUMAN

	Western blot analysis of extracts of various cell lines, using UBE2J1 antibody (CAB15841) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunofluorescence analysis of C6 cells using UBE2J1 antibody (CAB15841) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of HeLa cells using UBE2J1 antibody (CAB15841) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH/3T3 cells using UBE2J1 antibody (CAB15841) at dilution of 1:100. Blue: DAPI for nuclear staining.