Cell Cycle Antibodies 1
Anti-UBE2I Antibody (CAB2193)
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- SKU:
- CAB2193
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Cycle
Description
| Antibody Name: | Anti-UBE2I Antibody |
| Antibody SKU: | CAB2193 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-158 of human UBE2I (NP_003336.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, LO2, A-549, U-251MG, MCF-7, Mouse spleen, Mouse kidney, Rat spleen |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-158 of human UBE2I (NP_003336.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSGI ALSR LAQE RKAW RKDH PFGF VAVP TKNP DGTM NLMN WECA IPGK KGTP WEGG LFKL RMLF KDDY PSSP PKCK FEPP LFHP NVYP SGTV CLSI LEED KDWR PAIT IKQI LLGI QELL NEPN IQDP AQAE AYTI YCQN RVEY EKRV RAQA KKFA PS |
| Gene ID: | 7329 |
| Uniprot: | P63279 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 18kDa |
| Observed MW: | 18kDa |
| Synonyms: | UBE2I, C358B7.1, P18, UBC9 |
| Background: | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. |
| UniProt Protein Function: | Function: E2 ubiquitin-like--protein ligase mediating SUMO/Smt3 attachment to septins and PCNA. Seems to be involved in degradation of S- (CLB5) and M-phase cyclins (CLB2). Ref.4 Ref.5 |
| UniProt Protein Details: | Catalytic activity: ATP + SUMO + protein lysine = AMP + diphosphate + protein N-SUMOyllysine. Pathway: Protein modification; protein sumoylation. Subunit structure: Interacts with SIZ1. Ref.5 Ref.6 Subcellular location: Nucleus Ref.7. Miscellaneous: Present with 2600 molecules/cell in log phase SD medium. Ref.8 Sequence similarities: Belongs to the ubiquitin-conjugating enzyme family. |
| UniProt Code: | P63279 |
| NCBI GenInfo Identifier: | |
| NCBI Gene ID: | |
| NCBI Accession: | |
| Molecular Weight: | 17,911 Da |
| NCBI Full Name: | UBC9 |
| UniProt Protein Name: | SUMO-conjugating enzyme UBC9 |
| UniProt Synonym Protein Names: | Ubiquitin carrier protein 9; Ubiquitin-conjugating enzyme E2-18 kDa; Ubiquitin-protein ligase |
| UniProt Gene Name: | UBC9 |
| UniProt Entry Name: | UBC9_YEAST |
 | Western blot analysis of extracts of various cell lines, using UBE2I antibody (CAB2193) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded rat brain using UBE2I antibody (CAB2193) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse kidney using UBE2I antibody (CAB2193) at dilution of 1:100 (40x lens). |
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