Antibody Name:	Anti-UBE2G2 Antibody
Antibody SKU:	CAB10408
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-165 of human UBE2G2 (NP_003334.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, DU145, HT-1080, Mouse heart, Mouse testis, Rat heart, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-165 of human UBE2G2 (NP_003334.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAGT ALKR LMAE YKQL TLNP PEGI VAGP MNEE NFFE WEAL IMGP EDTC FEFG VFPA ILSF PLDY PLSP PKMR FTCE MFHP NIYP DGRV CISI LHAP GDDP MGYE SSAE RWSP VQSV EKIL LSVV SMLA EPND ESGA NVDA SKMW RDDR EQFY KIAK QIVQ KSLG L
Gene ID:	7327
Uniprot:	P60604
Cellular Location:
Calculated MW:	15kDa/18kDa
Observed MW:	19kDa

Synonyms:

UBE2G2, UBC7

Background:

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 100% sequence identity with the mouse counterpart. This gene is ubiquitously expressed, with high expression seen in adult muscle. Three alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

UniProt Protein Function:	UBE2G2: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 48'-linked polyubiquitination. Involved in endoplasmic reticulum- associated degradation (ERAD). Belongs to the ubiquitin-conjugating enzyme family.
UniProt Protein Details:	Protein type:EC 6.3.2.19; Endoplasmic reticulum; Ligase; Ubiquitin conjugating system; Ubiquitin ligase Chromosomal Location of Human Ortholog: 21q22.3 Cellular Component: cytosol Molecular Function:protein binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity Biological Process: cellular protein catabolic process; ER-associated protein catabolic process; protein amino acid N-linked glycosylation via asparagine; protein ubiquitination; ubiquitin-dependent protein catabolic process
NCBI Summary:	The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 100% sequence identity with the mouse counterpart. This gene is ubiquitously expressed, with high expression seen in adult muscle. Three alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jan 2011]
UniProt Code:	P60604
NCBI GenInfo Identifier:	45593583
NCBI Gene ID:	7327
NCBI Accession:	P60604.1
UniProt Secondary Accession:	P60604,P56554, A6NMQ7, A8K3L4, D3DSL7,
UniProt Related Accession:	P60604
Molecular Weight:	19kDa
NCBI Full Name:	Ubiquitin-conjugating enzyme E2 G2
NCBI Synonym Full Names:	ubiquitin conjugating enzyme E2 G2
NCBI Official Symbol:	UBE2G2
NCBI Official Synonym Symbols:	UBC7
NCBI Protein Information:	ubiquitin-conjugating enzyme E2 G2
UniProt Protein Name:	Ubiquitin-conjugating enzyme E2 G2
UniProt Synonym Protein Names:	E2 ubiquitin-conjugating enzyme G2; Ubiquitin carrier protein G2; Ubiquitin-protein ligase G2
Protein Family:	Ubiquitin-conjugating enzyme
UniProt Gene Name:	UBE2G2

	Western blot analysis of extracts of various cell lines, using UBE2G2 antibody (CAB10408) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of NIH-3T3 cells using UBE2G2 antibody (CAB10408) at dilution of 1:100. Blue: DAPI for nuclear staining.