Signal Transduction Antibodies 2
Anti-UBE2F Antibody (CAB5160)
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- SKU:
- CAB5160
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Signal Transduction
Description
| Antibody Name: | Anti-UBE2F Antibody |
| Antibody SKU: | CAB5160 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-185 of human UBE2F (NP_542409.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | K-562, Mouse liver, Mouse heart, Mouse pancreas, Mouse kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-185 of human UBE2F (NP_542409.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MLTL ASKL KRDD GLKG SRTA ATAS DSTR RVSV RDKL LVKE VAEL EANL PCTC KVHF PDPN KLHC FQLT VTPD EGYY QGGK FQFE TEVP DAYN MVPP KVKC LTKI WHPN ITET GEIC LSLL REHS IDGT GWAP TRTL KDVV WGLN SLFT DLLN FDDP LNIE AAEH HLRD KEDF RNKV DDYI KRYA R |
| Gene ID: | 140739 |
| Uniprot: | Q969M7 |
| Cellular Location: | |
| Calculated MW: | 11kDa/13kDa/17kDa/18kDa/21kDa/23kDa |
| Observed MW: | 21kDa |
| Synonyms: | UBE2F, NCE2 |
| Background: |
| UniProt Protein Function: | UBE2F: Accepts the ubiquitin-like protein NEDD8 from the UBA3- NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5. Belongs to the ubiquitin-conjugating enzyme family. UBE2F subfamily. 5 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 6.3.2.19; Ubiquitin ligase; EC 6.3.2.-; Ligase; Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 2q37.3 Cellular Component: cytoplasm Molecular Function:protein binding; ubiquitin protein ligase binding; ATP binding; NEDD8 ligase activity; ligase activity Biological Process: protein neddylation; protein ubiquitination |
| UniProt Code: | Q969M7 |
| NCBI GenInfo Identifier: | 74751725 |
| NCBI Gene ID: | 140739 |
| NCBI Accession: | Q969M7.1 |
| UniProt Related Accession: | Q969M7 |
| Molecular Weight: | Predicted Molecular Mass: 24.8kDaAccurate Molecular Mass: 25kDa |
| NCBI Full Name: | NEDD8-conjugating enzyme UBE2F |
| NCBI Synonym Full Names: | ubiquitin conjugating enzyme E2 F (putative) |
| NCBI Official Symbol: | UBE2F |
| NCBI Official Synonym Symbols: | NCE2 |
| NCBI Protein Information: | NEDD8-conjugating enzyme UBE2F |
| UniProt Protein Name: | NEDD8-conjugating enzyme UBE2F |
| UniProt Synonym Protein Names: | NEDD8 carrier protein UBE2F; NEDD8 protein ligase UBE2F; NEDD8-conjugating enzyme 2; Ubiquitin-conjugating enzyme E2 F |
| Protein Family: | NEDD8-conjugating enzyme |
| UniProt Gene Name: | UBE2F |
| UniProt Entry Name: | UBE2F_HUMAN |
 | Western blot analysis of extracts of various cell lines, using UBE2F antibody (CAB5160) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat ovary using UBE2F Rabbit pAb (CAB5160) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human liver cancer using UBE2F Rabbit pAb (CAB5160) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse heart using UBE2F Rabbit pAb (CAB5160) at dilution of 1:200 (40x lens). |
 | Immunofluorescence analysis of NIH-3T3 cells using UBE2F antibody (CAB5160) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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