Signal Transduction Antibodies 2

Anti-UBE2E2 Antibody (CAB16441)

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SKU:
CAB16441
Product Type:
Antibody
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-UBE2E2 Antibody
Antibody SKU:CAB16441
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-201 of human UBE2E2 (NP_689866.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse, Rat
Positive Samples:Mouse brain, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-201 of human UBE2E2 (NP_689866.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSTE AQRV DDSP STSG GSSD GDQR ESVQ QEPE REQV QPKK KEGK ISSK TAAK LSTS AKRI QKEL AEIT LDPP PNCS AGPK GDNI YEWR STIL GPPG SVYE GGVF FLDI TFSP DYPF KPPK VTFR TRIY HCNI NSQG VICL DILK DNWS PALT ISKV LLSI CSLL TDCN PADP LVGS IATQ YMTN RAEH DRMA RQWT KRYA T
Gene ID:7325
Uniprot:Q96LR5
Cellular Location:
Calculated MW:22kDa
Observed MW:22kDa
Synonyms:UBE2E2, UBCH8
Background:
UniProt Protein Function:UBE2E2: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 11'- and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. Belongs to the ubiquitin-conjugating enzyme family.
UniProt Protein Details:

Protein type:EC 6.3.2.19; Ubiquitin conjugating system; Ubiquitin ligase; Ligase

Chromosomal Location of Human Ortholog: 3p24.2

Molecular Function:ISG15 conjugating enzyme activity; protein binding; ubiquitin-protein ligase activity

Biological Process: ISG15-protein conjugation; response to DNA damage stimulus

UniProt Code:Q96LR5
NCBI GenInfo Identifier:47606201
NCBI Gene ID:7325
NCBI Accession:Q96LR5.1
UniProt Related Accession:Q96LR5
Molecular Weight:22,255 Da
NCBI Full Name:Ubiquitin-conjugating enzyme E2 E2
NCBI Synonym Full Names:ubiquitin conjugating enzyme E2 E2
NCBI Official Symbol:UBE2E2  
NCBI Official Synonym Symbols:UBCH8  
NCBI Protein Information:ubiquitin-conjugating enzyme E2 E2
UniProt Protein Name:Ubiquitin-conjugating enzyme E2 E2
UniProt Synonym Protein Names:E2 ubiquitin-conjugating enzyme E2; UbcH8; Ubiquitin carrier protein E2; Ubiquitin-protein ligase E2
Protein Family:Ubiquitin-conjugating enzyme
UniProt Gene Name:UBE2E2  
UniProt Entry Name:UB2E2_HUMAN
Anti-UBE2E2 Antibody (CAB16441)Western blot analysis of extracts of various cell lines, using UBE2E2 antibody (CAB16441) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 120s.
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