Epigenetics & Nuclear Signaling Antibodies 1
Anti-UBE2A Antibody (CAB12528)
- SKU:
- CAB12528
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-UBE2A Antibody |
| Antibody SKU: | CAB12528 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-152 of human UBE2A (NP_003327.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | Mouse kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-152 of human UBE2A (NP_003327.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSTP ARRR LMRD FKRL QEDP PAGV SGAP SENN IMVW NAVI FGPE GTPF EDGT FKLT IEFT EEYP NKPP TVRF VSKM FHPN VYAD GSIC LDIL QNRW SPTY DVSS ILTS IQSL LDEP NPNS PANS QAAQ LYQE NKRE YEKR VSAI VEQS WRDC |
| Gene ID: | 7319 |
| Uniprot: | P49459 |
| Cellular Location: | |
| Calculated MW: | 8kDa/13kDa/17kDa |
| Observed MW: | 17kDa |
| Synonyms: | UBE2A, HHR6A, MRXS30, MRXSN, RAD6A, UBC2 |
| Background: | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, ubiquitin-conjugating enzymes, and ubiquitin-protein ligases. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair, and may play a role in transcriptional regulation. Mutations in this gene are associated with mental retardation. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | UBE2A: a ubiquitin-protein ligase, a member of the ubiquitin-conjugating enzyme family. Homologous to the yeast DNA repair gene RAD6. Interacts with RAD18. Required for postreplication repair of UV-damaged DNA. |
| UniProt Protein Details: | Protein type:EC 6.3.2.19; Ubiquitin conjugating system; Ubiquitin ligase; Ligase Chromosomal Location of Human Ortholog: Xq24 Cellular Component: XY body; cytoplasm; nuclear chromatin; chromatin; cytosol Molecular Function:protein binding; small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity Biological Process: ubiquitin-dependent protein catabolic process; proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of peptide antigen via MHC class I; protein autoubiquitination; protein polyubiquitination; maternal process involved in pregnancy; postreplication repair; in utero embryonic development; positive regulation of cell proliferation; DNA repair; histone H2A ubiquitination; response to UV Disease: Mental Retardation, X-linked, Syndromic, Nascimento Type |
| UniProt Code: | P49459 |
| NCBI GenInfo Identifier: | 33518639 |
| NCBI Gene ID: | 7319 |
| NCBI Accession: | P49459.2 |
| Molecular Weight: | |
| NCBI Full Name: | Ubiquitin-conjugating enzyme E2 A |
| UniProt Protein Name: | Ubiquitin-conjugating enzyme E2 A |
| UniProt Synonym Protein Names: | RAD6 homolog A; HR6A; hHR6A; Ubiquitin carrier protein A; Ubiquitin-protein ligase A |
| Protein Family: | Ubiquitin-conjugating enzyme |
| UniProt Gene Name: | UBE2A |
| UniProt Entry Name: | UBE2A_HUMAN |
 | Western blot analysis of extracts of mouse kidney, using UBE2A antibody (CAB12528) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of U2OS cells using UBE2A antibody (CAB12528) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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