Signal Transduction Antibodies 3
Anti-UBA6 Antibody (CAB7511)
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- SKU:
- CAB7511
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Description
| Antibody Name: | Anti-UBA6 Antibody |
| Antibody SKU: | CAB7511 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF IP |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human UBA6 (NP_060697.4). |
| Application: | WB IHC IF IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:100 |
| Reactivity: | Human, Mouse |
| Positive Samples: | U-251MG, SKOV3, 293T, Mouse brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human UBA6 (NP_060697.4). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MEGS EPVA AHQG EEAS CSSW GTGS TNKN LPIM STAS VEID DALY SRQR YVLG DTAM QKMA KSHV FLSG MGGL GLEI AKNL VLAG IKAV TIHD TEKC QAWD LGTN FFLS EDDV VNKR NRAE AVLK HIAE LNPY VHVT SSSV PFNE TTDL SFLD KYQC VVLT EMKL PLQK KIND FCRS QCPP IKFI SADV HGIW SRLF CDFG DEFE VLDT TGEE PKEI FISN ITQA NPGI VTCL ENHP HKLE TGQF LTFR EING MTGL NGSI |
| Gene ID: | 55236 |
| Uniprot: | A0AVT1 |
| Cellular Location: | |
| Calculated MW: | 37kDa/43kDa/65kDa/117kDa |
| Observed MW: | 59kDa, 118kDa |
| Synonyms: | UBA6, E1-L2, MOP-4, UBE1L2 |
| Background: |
| UniProt Protein Function: | UBE1L2: Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin- E1 thioester and free AMP. Specific for ubiquitin, does not activate ubiquitin-like peptides. Differs from UBE1 in its specificity for substrate E2 charging. Does not charge cell cycle E2s, such as CDC34. Essential for embryonic development. Required for UBD/FAT10 conjugation. Isoform 2 may play a key role in ubiquitin system and may influence spermatogenesis and male fertility. Belongs to the ubiquitin-activating E1 family. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Ubiquitin ligase; EC 6.3.2.19; Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 4q13.2 Cellular Component: cytoplasm; cytosol Molecular Function:protein binding; FAT10 activating enzyme activity; ATP binding Biological Process: ubiquitin-dependent protein catabolic process; protein ubiquitination |
| NCBI Summary: | Modification of proteins with ubiquitin (UBB; MIM 191339) or ubiquitin-like proteins controls many signaling networks and requires a ubiquitin-activating enzyme (E1), a ubiquitin conjugating enzyme (E2), and a ubiquitin protein ligase (E3). UBE1L2 is an E1 enzyme that initiates the activation and conjugation of ubiquitin-like proteins (Jin et al., 2007 [PubMed 17597759]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | A0AVT1 |
| NCBI GenInfo Identifier: | 121949450 |
| NCBI Gene ID: | 55236 |
| NCBI Accession: | A0AVT1.1 |
| UniProt Related Accession: | A0AVT1 |
| Molecular Weight: | |
| NCBI Full Name: | Ubiquitin-like modifier-activating enzyme 6 |
| NCBI Synonym Full Names: | ubiquitin like modifier activating enzyme 6 |
| NCBI Official Symbol: | UBA6 |
| NCBI Official Synonym Symbols: | E1-L2; MOP-4; UBE1L2 |
| NCBI Protein Information: | ubiquitin-like modifier-activating enzyme 6 |
| UniProt Protein Name: | Ubiquitin-like modifier-activating enzyme 6 |
| UniProt Synonym Protein Names: | Monocyte protein 4; MOP-4; Ubiquitin-activating enzyme E1-like protein 2; E1-L2 |
| Protein Family: | Ubiquitin-like modifier-activating enzyme |
| UniProt Gene Name: | UBA6 |
| UniProt Entry Name: | UBA6_HUMAN |
 | Western blot analysis of extracts of various cell lines, using UBA6 antibody (CAB7511) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded human kidney using UBA6 antibody (CAB7511) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of A549 cells using UBA6 antibody (CAB7511). Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug UBA6 antibody (CAB7511). Western blot was performed from the immunoprecipitate using UBA6 antibody (CAB7511) at a dilition of 1:1000. |
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