Description
Antibody Name: | Anti-UBA52 Antibody (CAB20876) |
Antibody SKU: | CAB20876 |
Antibody Size: | 50µL, 100µL |
Application: | Western blotting, Immunohistochemistry, Immunofluorescence |
Reactivity: | Human, Mouse, Rat |
Host Species: | Rabbit |
Immunogen: | Recombinant protein of Human UBA52. |
Application: | Western blotting, Immunohistochemistry, Immunofluorescence |
Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
Reactivity: | Human, Mouse, Rat |
Positive Samples: | MCF7, PC-3, U-937, Mouse liver, Mouse testis, Mouse kidney, Rat liver, Rat testis |
Immunogen: | Recombinant protein of Human UBA52. |
Purification Method: | Affinity purification |
Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
Isotype: | IgG |
Sequence: | Email for sequence |
Cellular Location: | |
Calculated MW: | 15kDa |
Observed MW: | 8KDa |
Synonyms: | UBA52, CEP52, HUBCEP52, L40, RPL40 |
Background: | Ubiquitin is a highly conserved nuclear and cytoplasmic protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein L40 at the C terminus, a C-terminal extension protein (CEP). Multiple processed pseudogenes derived from this gene are present in the genome. |
Immunofluorescence analysis of PC-12 cells using UBA52 Rabbit mAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining. | |
Immunofluorescence analysis of U2OS cells using UBA52 Rabbit mAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining. | |
Immunofluorescence analysis of NIH/3T3 cells using UBA52 Rabbit mAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining. | |
Immunofluorescence analysis of HeLa cells using UBA52 Rabbit mAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining. | |
Immunohistochemistry of paraffin-embedded rat ovary using UBA52 Rabbit mAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. | |
Immunohistochemistry of paraffin-embedded mouse liver using UBA52 Rabbit mAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. | |
Immunohistochemistry of paraffin-embedded human brain using UBA52 Rabbit mAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. |